RANDOM MATING IN A NATURAL-POPULATION OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM

H A BABIKER, L C RANFORDCARTWRIGHT, D CURRIE, J D CHARLWOOD, P BILLINGSLEY, T TEUSCHER, D WALLIKER

Research output: Contribution to journalArticlepeer-review

173 Citations (Scopus)

Abstract

The genetic structure of a population of the malaria parasite Plasmodium falciparum has been examined in a village in Tanzania. Seventeen alleles of the merozoite surface protein MSP-1 and 23 of MSP-2 were detected by the polymerase chain reaction (PCR) among the blood parasites of the inhabitants. Most infections contained mixtures of genetically distinct parasite clones. PCR was then used to examine individual P. falciparum oocysts, the products of fertilization events, in wild-caught mosquitoes. Forty-five out of 71 oocysts were heterozygous for one or both genes, showing that crossing between clones was taking place frequently, following uptake of mixtures of gametocytes by the mosquitoes. The frequency of heterozygous forms showed that random mating events probably occurred within mosquito bloodmeals between gametes belonging to different parasite clones.

Original languageEnglish
Pages (from-to)413-421
Number of pages9
JournalParasitology
Volume109
Publication statusPublished - Nov 1994

Keywords

  • CROSS-FERTILIZATION
  • PLASMODIUM FALCIPARUM
  • MALARIA
  • OOCYSTS
  • MEROZOITE SURFACE-ANTIGEN
  • ANOPHELES-GAMBIAE SL
  • GENETIC DIVERSITY
  • POLYMORPHISM
  • AREA
  • DNA
  • AMPLIFICATION
  • PROTEINS
  • ALLELES

Fingerprint

Dive into the research topics of 'RANDOM MATING IN A NATURAL-POPULATION OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM'. Together they form a unique fingerprint.

Cite this