Quantitative spectroscopy of single molecule interaction times

H. H. Boltz*, A. Sirbu, N. Stelzer, M. J. Lohse, C. Schütte, P. Annibale*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
8 Downloads (Pure)


Single molecule fluorescence tracking provides information at nanometer-scale and millisecond-temporal resolution about the dynamics and interaction of individual molecules in a biological environment. While the dynamic behavior of isolated molecules can be characterized well, the quantitative insight is more limited when interactions between two indistinguishable molecules occur. We address this aspect by developing a theoretical foundation for a spectroscopy of interaction times, i.e., the inference of interaction from imaging data. A non-trivial crossover between a power law to an exponential behavior of the distribution of the interaction times is highlighted, together with the dependence of the exponential term upon the microscopic reaction affinity. Our approach is validated with simulated and experimental datasets.

Original languageEnglish
Pages (from-to)1538-1541
Number of pages4
JournalOptics Letters
Issue number7
Early online date19 Mar 2021
Publication statusPublished - 1 Apr 2021


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