TY - JOUR
T1 - Quantitative Photo Activated Localization Microscopy
T2 - Unraveling the effects of photoblinking
AU - Annibale, Paolo
AU - Vanni, Stefano
AU - Scarselli, Marco
AU - Rothlisberger, Ursula
AU - Radenovic, Aleksandra
PY - 2011
Y1 - 2011
N2 - In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
AB - In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
UR - http://www.scopus.com/inward/record.url?scp=79960806755&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0022678
DO - 10.1371/journal.pone.0022678
M3 - Article
C2 - 21818365
AN - SCOPUS:79960806755
SN - 1932-6203
VL - 6
JO - PLoS ONE
JF - PLoS ONE
IS - 7
M1 - e22678
ER -