Quantifying viable M. tuberculosis safely obviating the need for high containment facilities

Bariki Mtafya; Emmanuel Musisi; Paschal Qwaray; Emanuel Sichone; Natasha Walbaum; Nyanda Elias Ntinginya; Stephen H Gillespie; Wilber Sabiiti

doi:10.1007/978-1-0716-3981-8_14

Quantifying viable M. tuberculosis safely obviating the need for high containment facilities

Bariki Mtafya, Emmanuel Musisi, Paschal Qwaray, Emanuel Sichone, Natasha Walbaum, Nyanda Elias Ntinginya, Stephen H Gillespie, Wilber Sabiiti^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Mycobacterium tuberculosis is an infectious pathogen that requires biosafety level-3 laboratory for handling. The risk of transmission is high to laboratory staff, and to manage the organism safely, it is necessary to construct high containment laboratory facilities at great expense. This limits the application of tuberculosis diagnostics to areas where there is insufficient capital to invest in laboratory infrastructure. In this method, we describe a process of inactivating sputum samples by either heat or guanidine thiocyanate (GTC) that renders them safe without affecting the quantification of viable bacteria. This method eliminates the need for level 3 containment laboratory for the tuberculosis molecular bacterial load assay (TB-MBLA) and is applicable in low- and middle-income countries.

Original language	English
Title of host publication	Antibiotic resistance protocols
Editors	Stephen H. Gillespie
Place of Publication	New York
Publisher	Humana Press
Pages	145-152
Number of pages	8
Edition	4
ISBN (Electronic)	9781071639818
ISBN (Print)	9781071639801
DOIs	https://doi.org/10.1007/978-1-0716-3981-8_14
Publication status	Published - 2 Jul 2024

Publication series

Name	Methods in molecular biology
Publisher	Humana Press
Volume	2833
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Mycobacterium tuberculosis/isolation & purification
Humans
Thiocyanates
Containment of biohazards/methods
Sputum/microbiology
Bacterial load/methods
Tuberculosis/diagnosis
Guanidines
Hot temperature
Microbial viability

Access to Document

10.1007/978-1-0716-3981-8_14

Cite this

Mtafya, B., Musisi, E., Qwaray, P., Sichone, E., Walbaum, N., Ntinginya, N. E., Gillespie, S. H., & Sabiiti, W. (2024). Quantifying viable M. tuberculosis safely obviating the need for high containment facilities. In S. H. Gillespie (Ed.), Antibiotic resistance protocols (4 ed., pp. 145-152). (Methods in molecular biology; Vol. 2833). Humana Press. https://doi.org/10.1007/978-1-0716-3981-8_14

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Mtafya, B, Musisi, E, Qwaray, P, Sichone, E, Walbaum, N, Ntinginya, NE, Gillespie, SH & Sabiiti, W 2024, Quantifying viable M. tuberculosis safely obviating the need for high containment facilities. in SH Gillespie (ed.), Antibiotic resistance protocols. 4 edn, Methods in molecular biology, vol. 2833, Humana Press, New York, pp. 145-152. https://doi.org/10.1007/978-1-0716-3981-8_14

Quantifying viable M. tuberculosis safely obviating the need for high containment facilities. / Mtafya, Bariki; Musisi, Emmanuel; Qwaray, Paschal et al.
Antibiotic resistance protocols. ed. / Stephen H. Gillespie. 4. ed. New York: Humana Press, 2024. p. 145-152 (Methods in molecular biology; Vol. 2833).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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AU - Mtafya, Bariki

AU - Musisi, Emmanuel

AU - Qwaray, Paschal

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AU - Walbaum, Natasha

AU - Ntinginya, Nyanda Elias

AU - Gillespie, Stephen H

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KW - Guanidines

KW - Hot temperature

KW - Microbial viability

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