Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming

Richard Edward Randall; D Young; T Hanke; P Szawlowski; Catherine Helen Botting

Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming

Richard Edward Randall, D Young, T Hanke, P Szawlowski, Catherine Helen Botting

Research output: Contribution to journal › Article › peer-review

Abstract

This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes containing the p17, p27, vpr and vpx proteins of simian immunodeficiency virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modified for the manufacture of immune complexes for incorporation into multivalent vaccines.

Original language	English
Pages (from-to)	351-8
Number of pages	8
Journal	Vaccine
Volume	12
Issue number	4
Publication status	Published - Mar 1994

Keywords

SIV
VACCINE
SMAA COMPLEXES
ANTIBODY-ANTIGEN COMPLEXES
MONOCLONAL-ANTIBODIES
ACTIVATION
ADSORBENT
CELLS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming",

abstract = "This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes containing the p17, p27, vpr and vpx proteins of simian immunodeficiency virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modified for the manufacture of immune complexes for incorporation into multivalent vaccines.",

keywords = "SIV, VACCINE, SMAA COMPLEXES, ANTIBODY-ANTIGEN COMPLEXES, MONOCLONAL-ANTIBODIES, ACTIVATION, ADSORBENT, CELLS",

author = "Randall, {Richard Edward} and D Young and T Hanke and P Szawlowski and Botting, {Catherine Helen}",

year = "1994",

month = mar,

language = "English",

volume = "12",

pages = "351--8",

journal = "Vaccine",

issn = "0264-410X",

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T1 - Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming

AU - Randall, Richard Edward

AU - Young, D

AU - Hanke, T

AU - Szawlowski, P

AU - Botting, Catherine Helen

PY - 1994/3

Y1 - 1994/3

N2 - This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes containing the p17, p27, vpr and vpx proteins of simian immunodeficiency virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modified for the manufacture of immune complexes for incorporation into multivalent vaccines.

AB - This paper describes a general procedure for the two-step purification of recombinant proteins as antibody-antigen complexes in which there is no uncomplexed antibody or antigen. In this way, immune complexes containing the p17, p27, vpr and vpx proteins of simian immunodeficiency virus (SIV) have been purified. Antibody-antigen complexes are more immunogenic than antigen when administered either alone or with alum. The significance of the work is that this general method could be modified for the manufacture of immune complexes for incorporation into multivalent vaccines.

KW - SIV

KW - VACCINE

KW - SMAA COMPLEXES

KW - ANTIBODY-ANTIGEN COMPLEXES

KW - MONOCLONAL-ANTIBODIES

KW - ACTIVATION

KW - ADSORBENT

KW - CELLS

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M3 - Article

SN - 0264-410X

VL - 12

SP - 351

EP - 358

JO - Vaccine

JF - Vaccine

IS - 4

ER -

Purification of antibody-antigen complexes containing recombinant SIV proteins: comparison of antigen and antibody-antigen complexes for immune priming

Abstract

Keywords

UN SDGs

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