Projects per year
Abstract
The beta 1,2-rhamnosyltransferase WsaF is involved in the biosynthesis of a polyrhamnan chain which is attached to the surface-layer protein from Geobacillus stearothermophilus NRS 2004/3a. The enzyme belongs to the large retaining GT4 family. To date, no structure of a rhamnosyltransferase has been published. Recombinant purified native WsaF has been crystallized, resulting in crystals that belonged to space group P2(1)2(1)2(1) with unit-cell parameters a = 50.5, b = 56.1, c = 276.8 angstrom and diffracted to 3.0 angstrom resolution. Selenomethionine-variant WsaF crystallized in space group P2(1) with unit-cell parameters a = 75.9, b = 75.5, c = 78.1 angstrom and diffracted to 2.3 angstrom resolution.
Original language | English |
---|---|
Pages (from-to) | 1163-1165 |
Number of pages | 3 |
Journal | Acta Crystallographica. Section F, Structural biology and crystallization communications |
Volume | 64 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2008 |
Keywords
- S-LAYER GLYCOPROTEIN
- NRS 2004/3A
- FAMILY GT4
- GLYCOSYLTRANSFERASES
- GLYCOSYLATION
- BIOSYNTHESIS
- CATALYSIS
- INSIGHTS
Fingerprint
Dive into the research topics of 'Purification, crystallization and preliminary crystallographic analysis of WsaF, an essential rhamnosyltransferase from Geobacillus stearothermophilus'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Carb Export: Bacteria Ref: 081862/Z/06/Z: Carbohydrate export in bacteria
Naismith, J. (PI) & Ingledew, W. J. (CoI)
1/10/07 → 31/01/14
Project: Standard