Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase

Stephen A. McMahon, Muse Oke, Huanting Liu, Kenneth A. Johnson, Lester Carter, Nadia Kadi, Malcolm Frederick White, Gregory L. Challis, James Henderson Naismith

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3 Citations (Scopus)

Abstract

AcsD, a type A siderophore synthetase with a molecular weight of 71 140 Da from Pectobacterium chrysanthemi, has been expressed, purified and crystallized at 293 K. The protein crystallized in the primitive orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 80.3, b = 95.7, c = 161.1 angstrom, alpha = beta = gamma = 90 degrees. Systematic absences were consistent with space group P2(1)2(1)2(1). A complete data set has been collected to 2.25 angstrom resolution on BM14 at the ESRF. Consideration of the likely solvent content suggested that the asymmetric unit contained two molecules. Gel-filtration experiments indicated that the protein was a dimer, although self-rotation analyses did not detect a convincing twofold symmetry axis in the asymmetric unit. The protein has no convincing sequence match to any known structure and thus solution is likely to require experimental phasing.
Original languageEnglish
Pages (from-to)1052-1055
Number of pages4
JournalActa Crystallographica. Section F, Structural biology and crystallization communications
Volume64
Issue numberPart 11
DOIs
Publication statusPublished - Nov 2008

Keywords

  • BIOSYNTHESIS
  • IRON

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  • BBSRC BBS/B/14426: SPORT

    Naismith, J.

    BBSRC

    18/10/0430/04/12

    Project: Standard

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