Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

J Seetharamappa; Muse Oke; Huanting Liu; Stephen McMahon; Kenneth Alan Johnson; Lester Carter; M Dorward; M Zawadzki; IM Overton; CA van Niekirk; S Graham; Catherine Helen Botting; Garry Lindsay Taylor; Malcolm Frederick White; GJ Barton; Peter John Coote; James Henderson Naismith

doi:10.1107/S1744309107020362

Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

J Seetharamappa, Muse Oke, Huanting Liu, Stephen McMahon, Kenneth Alan Johnson, Lester Carter, M Dorward, M Zawadzki, IM Overton, CA van Niekirk, S Graham, Catherine Helen Botting, Garry Lindsay Taylor, Malcolm Frederick White, GJ Barton, Peter John Coote, James Henderson Naismith

Research output: Contribution to journal › Article › peer-review

Abstract

Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin- resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 angstrom, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees. A complete data set has been collected to 2.3 angstrom resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular- replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli ( PDB code 1iho), which is 41% identical to Sar2676, as a search model.

Original language	English
Pages (from-to)	488-491
Number of pages	4
Journal	Acta Crystallographica. Section F, Structural biology and crystallization communications
Volume	63
Issue number	6
DOIs	https://doi.org/10.1107/S1744309107020362
Publication status	Published - Jun 2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

MYCOBACTERIUM-TUBERCULOSIS
CRYSTAL-STRUCTURE
ESCHERICHIA-COLI
BIOSYNTHESIS
SURVEILLANCE
BACTEREMIA

Access to Document

10.1107/S1744309107020362

Cite this

Seetharamappa, J., Oke, M., Liu, H., McMahon, S., Johnson, K. A., Carter, L., Dorward, M., Zawadzki, M., Overton, IM., van Niekirk, CA., Graham, S., Botting, C. H., Taylor, G. L., White, M. F., Barton, GJ., Coote, P. J., & Naismith, J. H. (2007). Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase. Acta Crystallographica. Section F, Structural biology and crystallization communications , 63(6), 488-491. https://doi.org/10.1107/S1744309107020362

@article{de83a0445a824202b95882b2abcce21f,

title = "Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.",

abstract = "Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin- resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 angstrom, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees. A complete data set has been collected to 2.3 angstrom resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular- replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli ( PDB code 1iho), which is 41\% identical to Sar2676, as a search model.",

keywords = "MYCOBACTERIUM-TUBERCULOSIS, CRYSTAL-STRUCTURE, ESCHERICHIA-COLI, BIOSYNTHESIS, SURVEILLANCE, BACTEREMIA",

author = "J Seetharamappa and Muse Oke and Huanting Liu and Stephen McMahon and Johnson, \{Kenneth Alan\} and Lester Carter and M Dorward and M Zawadzki and IM Overton and \{van Niekirk\}, CA and S Graham and Botting, \{Catherine Helen\} and Taylor, \{Garry Lindsay\} and White, \{Malcolm Frederick\} and GJ Barton and Coote, \{Peter John\} and Naismith, \{James Henderson\}",

year = "2007",

month = jun,

doi = "10.1107/S1744309107020362",

language = "English",

volume = "63",

pages = "488--491",

journal = "Acta Crystallographica. Section F, Structural biology and crystallization communications ",

issn = "1744-3091",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "6",

}

Seetharamappa, J, Oke, M, Liu, H, McMahon, S, Johnson, KA, Carter, L, Dorward, M, Zawadzki, M, Overton, IM, van Niekirk, CA, Graham, S, Botting, CH, Taylor, GL , White, MF, Barton, GJ, Coote, PJ & Naismith, JH 2007, 'Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.', Acta Crystallographica. Section F, Structural biology and crystallization communications , vol. 63, no. 6, pp. 488-491. https://doi.org/10.1107/S1744309107020362

Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase. / Seetharamappa, J; Oke, Muse; Liu, Huanting et al.
In: Acta Crystallographica. Section F, Structural biology and crystallization communications , Vol. 63, No. 6, 06.2007, p. 488-491.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

AU - Seetharamappa, J

AU - Oke, Muse

AU - Liu, Huanting

AU - McMahon, Stephen

AU - Johnson, Kenneth Alan

AU - Carter, Lester

AU - Dorward, M

AU - Zawadzki, M

AU - Overton, IM

AU - van Niekirk, CA

AU - Graham, S

AU - Botting, Catherine Helen

AU - Taylor, Garry Lindsay

AU - White, Malcolm Frederick

AU - Barton, GJ

AU - Coote, Peter John

AU - Naismith, James Henderson

PY - 2007/6

Y1 - 2007/6

N2 - Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin- resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 angstrom, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees. A complete data set has been collected to 2.3 angstrom resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular- replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli ( PDB code 1iho), which is 41% identical to Sar2676, as a search model.

AB - Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin- resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 angstrom, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees. A complete data set has been collected to 2.3 angstrom resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular- replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli ( PDB code 1iho), which is 41% identical to Sar2676, as a search model.

KW - MYCOBACTERIUM-TUBERCULOSIS

KW - CRYSTAL-STRUCTURE

KW - ESCHERICHIA-COLI

KW - BIOSYNTHESIS

KW - SURVEILLANCE

KW - BACTEREMIA

UR - https://www.scopus.com/pages/publications/34249947297

UR - http://ukpmc.ac.uk/abstract/MED/17554169

U2 - 10.1107/S1744309107020362

DO - 10.1107/S1744309107020362

M3 - Article

SN - 1744-3091

VL - 63

SP - 488

EP - 491

JO - Acta Crystallographica. Section F, Structural biology and crystallization communications

JF - Acta Crystallographica. Section F, Structural biology and crystallization communications

IS - 6

ER -

Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

Abstract

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Keywords

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BBSRC BBS/B/14426: SPORT

Cite this

Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Projects

BBSRC BBS/B/14426: SPORT

Cite this