Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin- resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 angstrom, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees. A complete data set has been collected to 2.3 angstrom resolution at the ESRF. Consideration of the likely solvent content suggested the asymmetric unit to contain four molecules. This has been confirmed by molecular- replacement phasing calculations, which give a solution with four monomers using a monomer of pantothenate synthetase from Escherichia coli ( PDB code 1iho), which is 41% identical to Sar2676, as a search model.