Purification and functional inactivation of the fission yeast MCMMCM-BP complex

Juan-juan Li, Jasmin Schnick, Jacqueline Hayles, Stuart A. MacNeill

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The MCM (mini-chromosome maintenance) complex is the core of the eukaryotic replicative helicase and comprises six proteins, Mcm2-Mcm7. In humans, a variant form of the complex has Mcm2 replaced by the MCM-BP protein. Recent results suggest that a similar complex exists in fission yeast with an essential role in DNA replication and cell cycle progression. Here, we describe the purification and subunit composition of the fission yeast MCMMcb1 complex. Using newly generated temperature-sensitive alleles, we show that loss of MCMMcb1 function leads to accumulation of DNA damage, checkpoint activation and cell cycle arrest, and provide evidence for a role for MCMMcb1 in meiosis.

Original languageEnglish
Pages (from-to)3850-3855
Number of pages6
JournalFEBS Letters
Volume585
Issue number24
DOIs
Publication statusPublished - 15 Dec 2011

Keywords

  • DNA replication
  • Fission yeast
  • MCM helicase
  • TANDEM AFFINITY PURIFICATION
  • DNA-REPLICATION CHECKPOINT
  • SCHIZOSACCHAROMYCES-POMBE
  • MCM2-7 HELICASE
  • PROTEIN FAMILY
  • IDENTIFICATION
  • GINS
  • MCM9
  • ACTIVATION
  • MODULES

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