Pumilio-based RNA in vivo imaging

Jens Tilsner

doi:10.1007/978-1-4939-1523-1_20

Pumilio-based RNA in vivo imaging

Research output: Contribution to journal › Article › peer-review

Abstract

Subcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.

Original language	English
Pages (from-to)	295-328
Journal	Methods in Molecular Biology
Volume	1217
Early online date	11 Sept 2014
DOIs	https://doi.org/10.1007/978-1-4939-1523-1_20
Publication status	Published - 2015

Keywords

RNA imaging
Pumilo homology domain
RNA binding protein
Fluorescence complementation
Protein engineering
Fluorescent protein
Fluorescence microscopy
Tobacco mosaic virus

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10.1007/978-1-4939-1523-1_20

CH19.Tilsner.manuscript
© 2015. Springer Science+Business Media New York. The original publication is available at www.springerlink.com
Accepted author manuscript, 1.12 MB

Cite this

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title = "Pumilio-based RNA in vivo imaging",

abstract = "Subcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.",

keywords = "RNA imaging, Pumilo homology domain, RNA binding protein, Fluorescence complementation, Protein engineering, Fluorescent protein, Fluorescence microscopy, Tobacco mosaic virus",

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AB - Subcellular, sequence-specific detection of RNA in vivo is a powerful tool to study the macromolecular transport that occurs through plasmodesmata. The RNA-binding domain of Pumilio proteins can be engineered to bind RNA sequences of choice and fused to fluorescent proteins for RNA imaging. This chapter describes the construction of a Pumilio-based imaging system to track the RNA of Tobacco mosaic virus in vivo, and practical aspects of RNA live-cell imaging.

KW - RNA imaging

KW - Pumilo homology domain

KW - RNA binding protein

KW - Fluorescence complementation

KW - Protein engineering

KW - Fluorescent protein

KW - Fluorescence microscopy

KW - Tobacco mosaic virus

U2 - 10.1007/978-1-4939-1523-1_20

DO - 10.1007/978-1-4939-1523-1_20

M3 - Article

VL - 1217

SP - 295

EP - 328

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Pumilio-based RNA in vivo imaging

Abstract

Keywords

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