Projects per year
Abstract
Self-assembly of protein monomers directed by metal ion coordination
constitutes a promising strategy for designing supramolecular
architectures complicated by the noncovalent interaction between
monomers. Herein, two pulse dipolar electron paramagnetic resonance
spectroscopy (PDS) techniques, pulse electron–electron double resonance
and relaxation-induced dipolar modulation enhancement, were
simultaneously employed to study the CuII-templated dimerization behavior of a model protein (Streptococcus
sp. group G, protein G B1 domain) in both phosphate and Tris-HCl
buffers. A cooperative binding model could simultaneously fit all data
and demonstrate that the cooperativity of protein dimerization across
α-helical double-histidine motifs in the presence of CuII is
strongly modulated by the buffer, representing a platform for highly
tunable buffer-switchable templated dimerization. Hence, PDS enriches
the family of techniques for monitoring binding processes, supporting
the development of novel strategies for bioengineering structures and
stable architectures assembled by an initial metal-templated
dimerization.
Original language | English |
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Pages (from-to) | 7847-7852 |
Journal | The Journal of Physical Chemistry Letters |
Volume | 13 |
Issue number | 33 |
Early online date | 17 Aug 2022 |
DOIs | |
Publication status | Published - 25 Aug 2022 |
Fingerprint
Dive into the research topics of 'Pulse dipolar electron paramagnetic resonance spectroscopy reveals buffer modulated cooperativity of metal templated protein dimerization'. Together they form a unique fingerprint.Projects
- 4 Finished
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Enabling Shaped Pulse Capability: Enabling Shaped Pulse Capability for Superior Biological Structural Determination Using EPR Spectroscopy.
Lovett, J. E. (PI), Bode, B. E. (CoI), Penedo, C. (CoI), Pitt, S. J. (CoI), Schwarz-Linek, U. (CoI), Smith, G. M. (CoI), Watson, A. J. B. (CoI) & White, M. (CoI)
1/10/20 → 30/09/21
Project: Standard
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ISS3 round 4 Bela Bode: ISSF3 round 4 - Bela Bode
Bode, B. E. (PI)
1/11/19 → 31/12/20
Project: Standard
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Understanding sensitivity gains in pulse: Understanding sensitivity gains in pulse EPR on multimeric membrane proteins
Bode, B. E. (PI) & Pliotas, C. (CoI)
14/05/19 → 18/02/22
Project: Standard
Datasets
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Pulse Dipolar Electron Paramagnetic Resonance Spectroscopy Reveals Buffer Modulated Cooperativity of Metal Templated Protein Dimerization (dataset)
Oranges, M. (Creator), Wort, J. (Creator), Fukushima, M. (Creator), Fusco, E. (Creator), Ackermann, K. (Creator) & Bode, B. E. (Creator), University of St Andrews, 30 Aug 2022
DOI: 10.17630/bacc1277-fbfb-440f-bbc2-303aa11cd7e5
Dataset
File