Propionibacterium olivae sp nov and Propionibacterium damnosum sp nov., isolated from spoiled packaged Spanish-style green olives

Helena Lucena-Padros, Juan M. Gonzalez, Belen Caballero-Guerrero, Jose Luis Ruiz-Barba, Antonio Maldonado-Barragan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44% with Propionibacterium acidipropionici NCFB 563 and 98.33 and 98.11 % with Propionibacterium microaerophilum M5(T), respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from P. acidipropionici NCFB 563 and P. microaerophilum M5(T). Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus Propionibacterium. Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus Propionibacterium, for which the names Propionibacterium olivae sp. nov. (type strain, IGBL1(T)=CECT 8061(T)=DSM 25436(T)) and Propionibacterium damnosum sp. nov. (type strain, IGBL13(T)=CECT 8062(T)=DSM 25450(T)) are proposed.

Original languageEnglish
Pages (from-to)2980-2985
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume64
DOIs
Publication statusPublished - Sept 2014

Keywords

  • LACTOBACILLUS-PENTOSUS
  • FLUOROMETRIC METHOD
  • SEQUENCE
  • BACTERIA
  • MICROORGANISMS

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