Properties of the major Zn2+-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier

Jin  Lu; Stephen J. Hierons; Swati Arya; Remi Fritzen; Sirilata Polepalli; Siavash Khazaipoul; Alan J. Stewart; Claudia A. Blindauer

doi:10.1039/D4CC06611A

Properties of the major Zn²⁺-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier

Jin Lu, Stephen J. Hierons, Swati Arya, Remi Fritzen, Sirilata Polepalli, Siavash Khazaipoul, Alan J. Stewart^*, Claudia A. Blindauer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The foetal plasma protein α-fetoprotein (AFP) harbours a high-affinity zinc binding site that is likely involved in transport and delivery of essential zinc during foetal development. Based on a recent electron microscopy structure of AFP and aided by biophysical studies on an AFP-derived peptide, we present a refined 5-coordinate model for this site.

Original language	English
Pages (from-to)	2099-2102
Number of pages	4
Journal	Chemical Communications
Volume	61
Issue number	10
Early online date	25 Dec 2024
DOIs	https://doi.org/10.1039/D4CC06611A
Publication status	Published - 4 Feb 2025

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10.1039/D4CC06611ALicence: CC BY

Lu-2025-Properties-of-the-major-ChemComms-61-2099-CCBY
Copyright © 2025 The Authors. Open Access. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Final published version, 881 KBLicence: CC BY

Bilateral BBSRC-FAPESP: Bilateral BBSRC-FAPESP: A "speciomic" toolkit to investigate fatty acid-mediated changes in plasma zinc speciation and their physiological effects
Stewart, A. J. (PI)
BBSRC
1/11/21 → 31/10/24
Project: Standard
A Quantitive framework linking: A Quantitive framework linking extracellular zinc speciation and cell uptake
Stewart, A. J. (PI)
The Leverhulme Trust
4/09/17 → 3/09/20
Project: Standard
Dynamics of Circulatory Zinc Handling: The Dynamics of Circulatory Zinc Handling and Transport
Stewart, A. J. (PI)
BBSRC
9/04/12 → 8/04/15
Project: Standard

Cite this

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title = "Properties of the major Zn2+-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier",

abstract = "The foetal plasma protein α-fetoprotein (AFP) harbours a high-affinity zinc binding site that is likely involved in transport and delivery of essential zinc during foetal development. Based on a recent electron microscopy structure of AFP and aided by biophysical studies on an AFP-derived peptide, we present a refined 5-coordinate model for this site.",

author = "Jin Lu and Hierons, {Stephen J.} and Swati Arya and Remi Fritzen and Sirilata Polepalli and Siavash Khazaipoul and Stewart, {Alan J.} and Blindauer, {Claudia A.}",

note = "Funding: This work was financially supported by the Leverhulme Trust (RPG-2017-214) and BBSRC (BB/J006467/1 and BB/V014684/1).",

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doi = "10.1039/D4CC06611A",

language = "English",

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T1 - Properties of the major Zn2+-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier

AU - Lu, Jin

AU - Hierons, Stephen J.

AU - Arya, Swati

AU - Fritzen, Remi

AU - Polepalli, Sirilata

AU - Khazaipoul, Siavash

AU - Stewart, Alan J.

AU - Blindauer, Claudia A.

N1 - Funding: This work was financially supported by the Leverhulme Trust (RPG-2017-214) and BBSRC (BB/J006467/1 and BB/V014684/1).

PY - 2025/2/4

Y1 - 2025/2/4

N2 - The foetal plasma protein α-fetoprotein (AFP) harbours a high-affinity zinc binding site that is likely involved in transport and delivery of essential zinc during foetal development. Based on a recent electron microscopy structure of AFP and aided by biophysical studies on an AFP-derived peptide, we present a refined 5-coordinate model for this site.

AB - The foetal plasma protein α-fetoprotein (AFP) harbours a high-affinity zinc binding site that is likely involved in transport and delivery of essential zinc during foetal development. Based on a recent electron microscopy structure of AFP and aided by biophysical studies on an AFP-derived peptide, we present a refined 5-coordinate model for this site.

U2 - 10.1039/D4CC06611A

DO - 10.1039/D4CC06611A

M3 - Article

SN - 1359-7345

VL - 61

SP - 2099

EP - 2102

JO - Chemical Communications

JF - Chemical Communications

IS - 10

ER -

Properties of the major Zn2+-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier

Abstract

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Projects

Bilateral BBSRC-FAPESP: Bilateral BBSRC-FAPESP: A "speciomic" toolkit to investigate fatty acid-mediated changes in plasma zinc speciation and their physiological effects

A Quantitive framework linking: A Quantitive framework linking extracellular zinc speciation and cell uptake

Dynamics of Circulatory Zinc Handling: The Dynamics of Circulatory Zinc Handling and Transport

Cite this

Properties of the major Zn²⁺-binding site of human alpha-fetoprotein, a potential foetal plasma zinc carrier