Production of interlukin-12 as a self-processing polypeptide.

PJ Chaplin, EB Camon, B Villarreal-Ramos, M Flint, Martin Denis Ryan, RA Collins

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Interleukin-12 (IL-12) is a heterodimeric cytokine composed of two disulfide-linked subunits (p40 and p35) encoded by separate genes. We used the apparent autocleavage property of a 2A peptide from the foot-and-mouth disease virus (FMDV) to express bovine (Bo) IL-12 as a self-processing polypeptide (p402Ap35). We demonstrate that 2A will mediate the cleavage of p402Ap35 into two separate subunits in a manner similar to that observed during the processing of the FMDV polypeptide. Furthermore, this 2A polypeptide encoded a functional heterodimer, which elicited activities associated with IL-12 in other species. We propose that this strategy of self-processing polypeptides may be used in many applications where the coordinated and stoichiometric expression of complex proteins is required.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalJournal of Interferon and Cytokine Research
Volume19
Publication statusPublished - Mar 1999

Keywords

  • CELL STIMULATORY FACTOR
  • IFN-GAMMA
  • HETERODIMERIC CYTOKINE
  • HUMAN-LYMPHOCYTES
  • IN-VIVO
  • T-CELLS
  • CLONING
  • IL-12
  • MICE
  • EXPRESSION

Fingerprint

Dive into the research topics of 'Production of interlukin-12 as a self-processing polypeptide.'. Together they form a unique fingerprint.

Cite this