Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate)

RA Billington; JW Thuring; Stuart John Conway; L Packman; AB Holmes; AA Genazzani

Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate)

RA Billington, JW Thuring, Stuart John Conway, L Packman, AB Holmes, AA Genazzani

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca-2divided by-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor. the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

Original language	English
Pages (from-to)	275-280
Number of pages	6
Journal	Biochemical Journal
Volume	378
Publication status	Published - 15 Feb 2004

Keywords

calcium signalling
dehydrogenase
fertilization
reduced nicotinic acid-adenine dinucleotide phosphate (reduced NAADP)
nicotinic acid-adenine dinucleotide phosphate
(NAADP)
sea urchin
CYCLIC ADP-RIBOSE
SEA-URCHIN EGGS
INOSITOL TRISPHOSPHATE
CA2+ RELEASE
MOBILIZES CA2+
FERTILIZATION
MESSENGER
ROLES
INACTIVATION
COORDINATION

Cite this

@article{eec7da4c812e44f58efd45fabeefe0bb,

title = "Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate)",

abstract = "The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca-2divided by-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor. the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.",

keywords = "calcium signalling, dehydrogenase, fertilization, reduced nicotinic acid-adenine dinucleotide phosphate (reduced NAADP), nicotinic acid-adenine dinucleotide phosphate, (NAADP), sea urchin, CYCLIC ADP-RIBOSE, SEA-URCHIN EGGS, INOSITOL TRISPHOSPHATE, CA2+ RELEASE, MOBILIZES CA2+, FERTILIZATION, MESSENGER, ROLES, INACTIVATION, COORDINATION",

author = "RA Billington and JW Thuring and Conway, {Stuart John} and L Packman and AB Holmes and AA Genazzani",

note = "Address is Department of Chemistry, University of Cambridge",

year = "2004",

month = feb,

day = "15",

language = "English",

volume = "378",

pages = "275--280",

journal = "Biochemical Journal",

issn = "0264-6021",

publisher = "Portland Press Ltd.",

}

TY - JOUR

T1 - Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate)

AU - Billington, RA

AU - Thuring, JW

AU - Conway, Stuart John

AU - Packman, L

AU - Holmes, AB

AU - Genazzani, AA

N1 - Address is Department of Chemistry, University of Cambridge

PY - 2004/2/15

Y1 - 2004/2/15

N2 - The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca-2divided by-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor. the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

AB - The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca-2divided by-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor. the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

KW - calcium signalling

KW - dehydrogenase

KW - fertilization

KW - reduced nicotinic acid-adenine dinucleotide phosphate (reduced NAADP)

KW - nicotinic acid-adenine dinucleotide phosphate

KW - (NAADP)

KW - sea urchin

KW - CYCLIC ADP-RIBOSE

KW - SEA-URCHIN EGGS

KW - INOSITOL TRISPHOSPHATE

KW - CA2+ RELEASE

KW - MOBILIZES CA2+

KW - FERTILIZATION

KW - MESSENGER

KW - ROLES

KW - INACTIVATION

KW - COORDINATION

UR - http://www.scopus.com/inward/record.url?scp=1542284630&partnerID=8YFLogxK

M3 - Article

SN - 0264-6021

VL - 378

SP - 275

EP - 280

JO - Biochemical Journal

JF - Biochemical Journal

ER -

Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate)

Abstract

Keywords

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