Production and characterisation of monoclonal antibodies to antigens from Xiphinema index

Q Chen; RH Curtis; F Lamberti; M Moens; DJF Brown; JT Jones

doi:10.1163/156854103769224359

Production and characterisation of monoclonal antibodies to antigens from Xiphinema index

Q Chen, RH Curtis, F Lamberti, M Moens, DJF Brown, JT Jones^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

When feeding, Xiphinema index induces remarkable cellular modifications at the feeding site in the root tips of its hosts, similar to those induced by root endoparasites such as Meloidogyne spp. The large size of X. index enables direct analysis of their proteins. Following immunisation of a mouse with macerated extracts of X. index bodies, a panel of monoclonal antibodies (MAbs) was obtained against this nematode. The MAbs were used in immunolocalisation studies and antibodies recognising epitopes present at the surface coat, inner cuticle, dorsal gland cell and dorsal duct, body wall muscle fibres, nervous system and reproductive system were identified. These antibodies provide useful tools for studying plant-nematode interactions.

Original language	English
Pages (from-to)	359-366
Number of pages	8
Journal	Nematology
Volume	5
Issue number	3
DOIs	https://doi.org/10.1163/156854103769224359
Publication status	Published - 2003

Keywords

dorsal duct
MAbs
pharyngeal bulb
reproductive system
surface coat
PLANT-PARASITIC NEMATODES
CARBOHYDRATE EPITOPES
TRICHODORIDAE THORNE
BODY CUTICLE
ULTRASTRUCTURE
IDENTIFICATION
PROTEINS
ROOT
CELL

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title = "Production and characterisation of monoclonal antibodies to antigens from Xiphinema index",

abstract = "When feeding, Xiphinema index induces remarkable cellular modifications at the feeding site in the root tips of its hosts, similar to those induced by root endoparasites such as Meloidogyne spp. The large size of X. index enables direct analysis of their proteins. Following immunisation of a mouse with macerated extracts of X. index bodies, a panel of monoclonal antibodies (MAbs) was obtained against this nematode. The MAbs were used in immunolocalisation studies and antibodies recognising epitopes present at the surface coat, inner cuticle, dorsal gland cell and dorsal duct, body wall muscle fibres, nervous system and reproductive system were identified. These antibodies provide useful tools for studying plant-nematode interactions.",

keywords = "dorsal duct, MAbs, pharyngeal bulb, reproductive system, surface coat, PLANT-PARASITIC NEMATODES, CARBOHYDRATE EPITOPES, TRICHODORIDAE THORNE, BODY CUTICLE, ULTRASTRUCTURE, IDENTIFICATION, PROTEINS, ROOT, CELL",

author = "Q Chen and RH Curtis and F Lamberti and M Moens and DJF Brown and JT Jones",

year = "2003",

doi = "10.1163/156854103769224359",

language = "English",

volume = "5",

pages = "359--366",

journal = "Nematology",

issn = "1388-5545",

publisher = "Brill",

number = "3",

}

TY - JOUR

T1 - Production and characterisation of monoclonal antibodies to antigens from Xiphinema index

AU - Chen, Q

AU - Curtis, RH

AU - Lamberti, F

AU - Moens, M

AU - Brown, DJF

AU - Jones, JT

PY - 2003

Y1 - 2003

N2 - When feeding, Xiphinema index induces remarkable cellular modifications at the feeding site in the root tips of its hosts, similar to those induced by root endoparasites such as Meloidogyne spp. The large size of X. index enables direct analysis of their proteins. Following immunisation of a mouse with macerated extracts of X. index bodies, a panel of monoclonal antibodies (MAbs) was obtained against this nematode. The MAbs were used in immunolocalisation studies and antibodies recognising epitopes present at the surface coat, inner cuticle, dorsal gland cell and dorsal duct, body wall muscle fibres, nervous system and reproductive system were identified. These antibodies provide useful tools for studying plant-nematode interactions.

AB - When feeding, Xiphinema index induces remarkable cellular modifications at the feeding site in the root tips of its hosts, similar to those induced by root endoparasites such as Meloidogyne spp. The large size of X. index enables direct analysis of their proteins. Following immunisation of a mouse with macerated extracts of X. index bodies, a panel of monoclonal antibodies (MAbs) was obtained against this nematode. The MAbs were used in immunolocalisation studies and antibodies recognising epitopes present at the surface coat, inner cuticle, dorsal gland cell and dorsal duct, body wall muscle fibres, nervous system and reproductive system were identified. These antibodies provide useful tools for studying plant-nematode interactions.

KW - dorsal duct

KW - MAbs

KW - pharyngeal bulb

KW - reproductive system

KW - surface coat

KW - PLANT-PARASITIC NEMATODES

KW - CARBOHYDRATE EPITOPES

KW - TRICHODORIDAE THORNE

KW - BODY CUTICLE

KW - ULTRASTRUCTURE

KW - IDENTIFICATION

KW - PROTEINS

KW - ROOT

KW - CELL

U2 - 10.1163/156854103769224359

DO - 10.1163/156854103769224359

M3 - Article

SN - 1388-5545

VL - 5

SP - 359

EP - 366

JO - Nematology

JF - Nematology

IS - 3

ER -

Production and characterisation of monoclonal antibodies to antigens from Xiphinema index

Abstract

Keywords

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