Production and characterisation of monoclonal antibodies to antigens from Xiphinema index

Q Chen, RH Curtis, F Lamberti, M Moens, DJF Brown, JT Jones*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

When feeding, Xiphinema index induces remarkable cellular modifications at the feeding site in the root tips of its hosts, similar to those induced by root endoparasites such as Meloidogyne spp. The large size of X. index enables direct analysis of their proteins. Following immunisation of a mouse with macerated extracts of X. index bodies, a panel of monoclonal antibodies (MAbs) was obtained against this nematode. The MAbs were used in immunolocalisation studies and antibodies recognising epitopes present at the surface coat, inner cuticle, dorsal gland cell and dorsal duct, body wall muscle fibres, nervous system and reproductive system were identified. These antibodies provide useful tools for studying plant-nematode interactions.

Original languageEnglish
Pages (from-to)359-366
Number of pages8
JournalNematology
Volume5
Issue number3
DOIs
Publication statusPublished - 2003

Keywords

  • dorsal duct
  • MAbs
  • pharyngeal bulb
  • reproductive system
  • surface coat
  • PLANT-PARASITIC NEMATODES
  • CARBOHYDRATE EPITOPES
  • TRICHODORIDAE THORNE
  • BODY CUTICLE
  • ULTRASTRUCTURE
  • IDENTIFICATION
  • PROTEINS
  • ROOT
  • CELL

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