Postprandial expression of growth-related genes in Atlantic salmon (Salmo salar L.) juveniles fasted for 1 week and fed a single meal to satiation

Luisa M. P. Valente*, Neil I. Bower, Ian A. Johnston

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

We investigated postprandial changes in transcript abundance following a single satiating meal in juvenile Atlantic salmon (Salmo salar L.) (about 70 g body mass) following fasting for 1 week at 12 degrees C. The expression of twenty-three growth-related genes was determined in fast myotomal muscle using quantitative real-time PCR at the following postprandial time points: -12, 0, 1, 3, 6, 12, 24, 48 and 96 h. The gut was fullest 1-6 h after feeding and emptied within 48-96 h. IGF-I, MyoD1c, MRF4 and myf5 transcripts were sharply up-regulated within 1 h of refeeding and are promising candidate genes involved in a fast-response signalling system that regulates fish myotomal muscle growth. These genes clustered together with MyoD1b and suggest a coordinated regulation to favour resumption of myogenesis as an early response to feeding. Insulin-like growth factor (IGF)-II and the ubiquitin ligase MAFbx/atrogin-1 were initially down-regulated but restored to initial values after 12 h. It is also suggested that local production of IGF-I within the muscle might suppress catabolic pathways depressing MAFbx/atrogin-1.

Original languageEnglish
Pages (from-to)2148-2157
Number of pages10
JournalBritish Journal of Nutrition
Volume108
Issue number12
DOIs
Publication statusPublished - 28 Dec 2012

Keywords

  • Salmo salar L.
  • Myogenesis
  • Refeeding and fasting
  • Nutrient availability
  • Insulin-like growth factor system
  • TROUT ONCORHYNCHUS-MYKISS
  • MESSENGER-RNA EXPRESSION
  • GILTHEAD SEA BREAM
  • RAINBOW-TROUT
  • COMPENSATORY GROWTH
  • SKELETAL-MUSCLE
  • FACTOR-I
  • IGF-I
  • SIGNALING PATHWAYS
  • REGULATORY FACTORS

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