Polynomial algebra reveals diverging roles of the unfolded protein response in endothelial cells during ischemia–reperfusion injury

Sylvain Le Pape, Elena Dimitrova, Patrick Hannaert, Alexander Konovalov, Romain Volmer, David Ron, Raphaël Thuillier, Thierry Hauet

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The unfolded protein response (UPR) – the endoplasmic reticulum stress response – is found in various pathologies including ischemia–reperfusion injury (IRI). However, its role during IRI is still unclear. Here, by combining two different bioinformatical methods – a method based on ordinary differential equations (Time Series Network Inference) and an algebraic method (probabilistic polynomial dynamical systems) – we identified the IRE1α–XBP1 and the ATF6 pathways as the main UPR effectors involved in cell’s adaptation to IRI. We validated these findings experimentally by assessing the impact of their knock-out and knock-down on cell survival during IRI.
Original languageEnglish
Pages (from-to)3062-3067
JournalFEBS Letters
Volume588
Issue number17
Early online date16 Jun 2014
DOIs
Publication statusPublished - 25 Aug 2014

Keywords

  • Ischemia–reperfusion injury (IRI)
  • Unfolded protein response (UPR)
  • Endothelial cells (EC)
  • Murine embryonic cells (MEC)
  • Probabilistic polynomial dynamical systems (PDS)
  • Gene regulatory networks (GRN)

Fingerprint

Dive into the research topics of 'Polynomial algebra reveals diverging roles of the unfolded protein response in endothelial cells during ischemia–reperfusion injury'. Together they form a unique fingerprint.

Cite this