Plasmodium chabaudi: a rodent malaria model for in‐vivo and in‐vitro cytoadherence of malaria parasites in the absence of knobs

J. COX*, S. SEMOFF, M. HOMMEL

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Summary The ability of Plasmodium chabaudi infected erythrocytes to bind to endothelial cells in vivo and to various murine cell lines in vitro is described. A procedure for the selective recovery of a sequestering subpopulation of schizont‐infected erythrocytes from hepatic sinusoids of BALB/c mice, using a combination of body perfusion, trypsin treatment and Percoll density centrifugation was developed. The serial subinoculation of such a subpopulation was used to select for a clone of parasites (strain AJJ) with considerably better cytoadherent characteristics than the parent line (strain AJ). In contrast, it was demonstrated that another clone (PC7), showed no cytoadherence in vivo or in vitro. This study shows that parasite induced alterations occurred on the surface of erythrocytes infected with late developmental stages of P. chabaudi. The antigenicity of these molecules in the infected mouse was demonstrated using immune serum and affinity chromatography. Cytoadherence and surface antigenic changes in P. chabaudi schizont‐infected erythrocytes were demonstrated in the absence of the submembranous ‘knobs’ associated with cytoadherence in P. falciparum.

Original languageEnglish
Pages (from-to)543-561
Number of pages19
JournalParasite Immunology
Volume9
Issue number5
DOIs
Publication statusPublished - 1987

Keywords

  • cerebral malaria
  • knobs
  • P. chabaudi
  • sequestration

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