Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

Shiela E Unkles; Vicki Frances Symington; Zorica Kotur; Ye Wang; Yaeesh Siddiqi; James Robertson Kinghorn; Anthony Glass

doi:10.1128/EC.05199-11

Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

Shiela E Unkles, Vicki Frances Symington, Zorica Kotur, Ye Wang, Yaeesh Siddiqi, James Robertson Kinghorn, Anthony Glass

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Abstract

High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.

Original language	English
Pages (from-to)	1724-1732
Number of pages	9
Journal	Eukaryotic Cell
Volume	10
Issue number	12
Early online date	21 Oct 2011
DOIs	https://doi.org/10.1128/EC.05199-11
Publication status	Published - Dec 2011

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title = "Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.",

abstract = "High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.",

author = "Unkles, {Shiela E} and Symington, {Vicki Frances} and Zorica Kotur and Ye Wang and Yaeesh Siddiqi and Kinghorn, {James Robertson} and Anthony Glass",

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T1 - Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

AU - Unkles, Shiela E

AU - Symington, Vicki Frances

AU - Kotur, Zorica

AU - Wang, Ye

AU - Siddiqi, Yaeesh

AU - Kinghorn, James Robertson

AU - Glass, Anthony

PY - 2011/12

Y1 - 2011/12

N2 - High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.

AB - High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.

UR - http://ukpmc.ac.uk/articles/PMC3232726

U2 - 10.1128/EC.05199-11

DO - 10.1128/EC.05199-11

M3 - Article

SN - 1535-9778

VL - 10

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EP - 1732

JO - Eukaryotic Cell

JF - Eukaryotic Cell

IS - 12

ER -

Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

Abstract

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