Physiological and biochemical characterization of AnNitA, the Aspergillus nidulans high-affinity nitrite transporter.

Shiela E Unkles, Vicki Frances Symington, Zorica Kotur, Ye Wang, Yaeesh Siddiqi, James Robertson Kinghorn, Anthony Glass

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

High-affinity nitrite influx into mycelia of Aspergillus nidulans has been characterized by use of (13)NO(2)(-), giving average K(m) and V(max) values of 48 ± 8 μM and 228 ± 49 nmol mg(-1) dry weight (DW) h(-1), respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (r(2) = 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.
Original languageEnglish
Pages (from-to)1724-1732
Number of pages9
JournalEukaryotic Cell
Volume10
Issue number12
Early online date21 Oct 2011
DOIs
Publication statusPublished - Dec 2011

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