Phenotypic resistance in mycobacteria: is it because I am old or fat that I resist you?

Robert J H Hammond, Vincent O Baron, Katarina Oravcova, Sam Lipworth, Stephen H Gillespie

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Synopsis
Objectives
We aimed to explore the phenomenon of phenotypic resistance to anti-mycobacterial antibiotics and to determine whether this was associated with cell age or presence of lipid bodies.

Methods
The accumulation of lipid body positive cells (lipid rich- LR) was followed using cell staining and flow cytometry. LR cells of M. smegmatis, M. marinum, M. fortuitum and BGC were separated from non-lipid body containing cells (lipid poor- LP) and their MBC determined. We also compared the MBC of LR and LP from “old” and “young” cultures.


Results
The LR cells of all species were more resistant to antibiotics than LP cells. For Mycobacterium bovis (BCG) the susceptibility ratios were as follows; Rifampicin-5X, isoniazid-16.7X, ethambutol-5X, ciprofloxacin-5X. Phenotypic resistance was found in LR cells irrespective of cell age.

Conclusions
We have shown that phenotypic antibiotic resistance is associated with the presence of lipid bodies irrespective of cell age. These data have important implications for our understanding of relapse in mycobacterial infections.

Original languageEnglish
JournalJournal of Antimicrobial Chemotherapy
VolumeAdvance Access
DOIs
Publication statusPublished - 9 Jul 2015

Keywords

  • Mycobacterium tuberculosis
  • Antibiotic resistance
  • Lipid rich
  • Lipid poor

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