Phagocytic activity of Limulus polyphemus amebocytes in vitro

Christopher J. Coates*, Tim Whalley, Jacqueline Nairn

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Phagocytosis of invading microorganisms is a fundamental component of innate immunity. The Atlantic horseshoe crab, Limulus polyphemus, possesses a single immune cell type, the granular amebocyte. Amebocytes release a repertoire of potent immune effectors in the presence of pathogens, and function in hemostasis. In contrast to other arthropod immunocytes, the properties of amebocyte phagocytosis remain poorly characterised, restricted by the technical challenges associated with handling these labile cells. We have addressed these challenges and observed the internalisation of microbial and synthetic targets by amebocytes in vitro. Confirmation of target internalisation was achieved using a combination of fluorescent quenching and lipophilic membrane probes: R18 and FM 1-43. Viability, morphological integrity and functionality of extracted amebocytes appeared to be retained in vitro. The phagocytic properties of L polyphemus amebocytes described here, in the absence of endotoxin, are similar to those observed for arthropod immunocytes and mammalian neutrophils. (c) 2012 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalJournal of Invertebrate Pathology
Volume111
Issue number3
DOIs
Publication statusPublished - Nov 2012

Keywords

  • Phagocytosis
  • Amebocytes
  • Limulus polyphemus
  • Cellular defence
  • Innate immunity
  • Fluorescent probes
  • INSECT-CELL CULTURE
  • HORSESHOE-CRAB
  • IMMUNE-RESPONSES
  • INNATE IMMUNITY
  • GROMPHADORHINA PORTENTOSA
  • PLASMA-MEMBRANE
  • HEMOCYTES
  • IMMUNOCYTES
  • NEUTROPHILS
  • ARTHROPODS

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