PCNA-binding proteins in the archaea: novel functionality beyond the conserved core

Stuart Andrew MacNeill

doi:10.1007/s00294-016-0577-3

PCNA-binding proteins in the archaea: novel functionality beyond the conserved core

Research output: Contribution to journal › Review article › peer-review

Abstract

Sliding clamps play an essential role in coordinating protein activity in DNA metabolism in all three domains of life. In eukaryotes and archaea the sliding clamp is PCNA (proliferating cell nuclear antigen). Across the diversity of the archaea PCNA interacts with a highly conserved set of proteins with key roles in DNA replication and repair, including DNA polymerases B and D, replication factor C, the Fen1 nuclease and RNAseH2, but this core set of factors is likely to represent a fraction of the PCNA interactome only. Here I review three recently characterised non-core archaeal PCNA binding proteins NusS, NreA/NreB and TIP, highlighting what is known of their interactions with PCNA and their functions in vivo and in vitro. Gaining a detailed understanding of the non-core PCNA interactome will provide significant insights into key aspects of chromosome biology in divergent archaeal lineages.

Original language	English
Pages (from-to)	527-532
Number of pages	6
Journal	Current Genetics
Volume	62
Issue number	3
Early online date	17 Feb 2016
DOIs	https://doi.org/10.1007/s00294-016-0577-3
Publication status	Published - Aug 2016

Keywords

Sliding clamp
Interactome
Chromosome biology
DNA repair
DNA replication
Archaea
PCNA

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10.1007/s00294-016-0577-3

MacNeill_2016_CurrGenet_CCBY
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Cite this

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title = "PCNA-binding proteins in the archaea: novel functionality beyond the conserved core",

abstract = "Sliding clamps play an essential role in coordinating protein activity in DNA metabolism in all three domains of life. In eukaryotes and archaea the sliding clamp is PCNA (proliferating cell nuclear antigen). Across the diversity of the archaea PCNA interacts with a highly conserved set of proteins with key roles in DNA replication and repair, including DNA polymerases B and D, replication factor C, the Fen1 nuclease and RNAseH2, but this core set of factors is likely to represent a fraction of the PCNA interactome only. Here I review three recently characterised non-core archaeal PCNA binding proteins NusS, NreA/NreB and TIP, highlighting what is known of their interactions with PCNA and their functions in vivo and in vitro. Gaining a detailed understanding of the non-core PCNA interactome will provide significant insights into key aspects of chromosome biology in divergent archaeal lineages.",

keywords = "Sliding clamp, Interactome, Chromosome biology, DNA repair, DNA replication , Archaea, PCNA",

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AB - Sliding clamps play an essential role in coordinating protein activity in DNA metabolism in all three domains of life. In eukaryotes and archaea the sliding clamp is PCNA (proliferating cell nuclear antigen). Across the diversity of the archaea PCNA interacts with a highly conserved set of proteins with key roles in DNA replication and repair, including DNA polymerases B and D, replication factor C, the Fen1 nuclease and RNAseH2, but this core set of factors is likely to represent a fraction of the PCNA interactome only. Here I review three recently characterised non-core archaeal PCNA binding proteins NusS, NreA/NreB and TIP, highlighting what is known of their interactions with PCNA and their functions in vivo and in vitro. Gaining a detailed understanding of the non-core PCNA interactome will provide significant insights into key aspects of chromosome biology in divergent archaeal lineages.

KW - Sliding clamp

KW - Interactome

KW - Chromosome biology

KW - DNA repair

KW - DNA replication

KW - Archaea

KW - PCNA

U2 - 10.1007/s00294-016-0577-3

DO - 10.1007/s00294-016-0577-3

M3 - Review article

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SP - 527

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JO - Current Genetics

JF - Current Genetics

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PCNA-binding proteins in the archaea: novel functionality beyond the conserved core

Abstract

Keywords

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