Parallel and comparative analysis of the proteome and transcriptome of sorbic acid-stressed Saccharomyces cerevisiae

H De Nobel, L Lawrie, S Brul, F Klis, M Davis, H Alloush, Peter John Coote

Research output: Contribution to journalArticlepeer-review

103 Citations (Scopus)

Abstract

Exposure of Saccharomyces cerevisiae to 0.9 mM sorbic acid at pH 4.5 resulted in the upregulation of 10 proteins; Hsp42, Atp2, Hsp26. Ssa1 or Ssa2, Ssb1 or Ssb2, Ssc1, Ssa4, Ach1, Zwf1 and Tdh1; and the downregulation of three proteins; Ade16, Adh3 and Eno2. In parallel, of 6144 ORFs. 94 (1.53%) showed greater than a 1.4-fold increase in transcript level after exposure to sorbic acid and five of these were increased greater than two-fold; MFA1, AGA2, HSP26, SIP18 and YDR533C. Similarly, of 6144 ORFs, 72 (1.17%) showed greater than a 1.4-fold decrease in transcript level and only one of these, PCK1, was decreased greater than two-fold. Functional categories of genes that were induced by sorbic acid stress included cell stress (particularly oxidative stress), transposon function, mating response and energy generation. We found that proteomic analysis yielded distinct information from transcript analysis. Only the upregulation of Hsp26 was detected by both methods. Subsequently, we demonstrated that a deletion mutant of Hsp26 was sensitive to sorbic acid. Thus, the induction of Hsp26, which occurs during adaptation to sorbic acid, confers resistance to the inhibitory effects of this compound. Copyright (C) 2001 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)1413-1428
Number of pages16
JournalYeast
Volume18
Issue number15
DOIs
Publication statusPublished - Nov 2001

Keywords

  • DNA microarrays
  • proteomics
  • stress
  • transcriptome
  • weak acid preservatives
  • HEAT-SHOCK-PROTEIN
  • PENTOSE-PHOSPHATE PATHWAY
  • MEMBRANE H+-ATPASE
  • GENE-EXPRESSION
  • HYDROGEN-PEROXIDE
  • OXIDATIVE STRESS
  • PLASMA-MEMBRANE
  • SUPEROXIDE-DISMUTASE
  • POLYACRYLAMIDE GELS
  • ABC TRANSPORTER

Fingerprint

Dive into the research topics of 'Parallel and comparative analysis of the proteome and transcriptome of sorbic acid-stressed Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this