TY - JOUR
T1 - Optimizing the performance of single-cell ICP-MS for Fe and Zn determination in human umbilical vascular endothelial cells
AU - Leal, Ketolly N. S.
AU - da Silva, Ana B. S.
AU - Aragão, Annelize Z. B.
AU - Ramos, Carlos H. I.
AU - Stewart, Alan J.
AU - Arruda, Marco A. Z.
N1 - Funding: This work was financial supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2014/50867-3, 2017/26131-5, 2018/25207-0, 2019/24445-8, 2020/06934-9, and 2020/08543-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq grant number 303231/2020-3, and the scholarchip SWE-CNPq 425 200475/2022-3) and BBSRC (Biological and Biotechnological Sciences Research Council). Grant No: BB/V014684/1.
PY - 2024/7/1
Y1 - 2024/7/1
N2 - Inductively coupled plasma-mass spectrometry operated in a single-cell mode (scICP-MS/MS) has emerged as a technique that allows measurement of metal concentration at the cellular level. The approach is made possible by the high sensitivity of modern instrumentation. However, specific applications may be limited by spectral and non-spectral interference that can occur due to the complexity of the matrix that requires to be analysed. As such, the success of this approach requires adequate sample preparation, allowing the stability of the cells to be preserved before introducing them into the plasma. In this context, a method was developed to measure total Zn and Fe concentrations in individual human umbilical vein endothelial cells (HUVECs) using scICP-MS/MS. As a strategy, the suspension of HUVECs was prefixed with paraformaldehyde aiming to achieve experimental stability and repeatability without significantly affecting the background signal. Moreover, different dwell times (3, 5 and 10 ms) were assessed to optimize the instrumental parameters. As a result, the best performance for Zn and Fe measurement by scICP-MS/MS in terms of sensitivity and accuracy was obtained with the dwell time of 3 ms. To the best of our knowledge, the concentrations of Fe and Zn in fixed single endothelial cells has not been explored previously using scICP-MS. Here an scICP-MS/MS method was developed and used successfully to examine the influence of different culture conditions on Fe and Zn uptake in fixed single HUVEC cells.
AB - Inductively coupled plasma-mass spectrometry operated in a single-cell mode (scICP-MS/MS) has emerged as a technique that allows measurement of metal concentration at the cellular level. The approach is made possible by the high sensitivity of modern instrumentation. However, specific applications may be limited by spectral and non-spectral interference that can occur due to the complexity of the matrix that requires to be analysed. As such, the success of this approach requires adequate sample preparation, allowing the stability of the cells to be preserved before introducing them into the plasma. In this context, a method was developed to measure total Zn and Fe concentrations in individual human umbilical vein endothelial cells (HUVECs) using scICP-MS/MS. As a strategy, the suspension of HUVECs was prefixed with paraformaldehyde aiming to achieve experimental stability and repeatability without significantly affecting the background signal. Moreover, different dwell times (3, 5 and 10 ms) were assessed to optimize the instrumental parameters. As a result, the best performance for Zn and Fe measurement by scICP-MS/MS in terms of sensitivity and accuracy was obtained with the dwell time of 3 ms. To the best of our knowledge, the concentrations of Fe and Zn in fixed single endothelial cells has not been explored previously using scICP-MS. Here an scICP-MS/MS method was developed and used successfully to examine the influence of different culture conditions on Fe and Zn uptake in fixed single HUVEC cells.
U2 - 10.1016/j.microc.2024.110696
DO - 10.1016/j.microc.2024.110696
M3 - Article
SN - 1095-9149
VL - 202
JO - Microchemical Journal
JF - Microchemical Journal
M1 - 110696
ER -