Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta

Philip Wijesinghe; Niloufer J. Johansen; Andrea Curatolo; David D. Sampson; Ruth Ganss; Brendan F. Kennedy

doi:10.1117/12.2269903

Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta

Philip Wijesinghe, Niloufer J. Johansen, Andrea Curatolo, David D. Sampson, Ruth Ganss, Brendan F. Kennedy

School of Physics and Astronomy

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have developed a high-resolution optical coherence elastography system capable of estimating Young's modulus in tissue volumes with an isotropic resolution of 15 μm over a 1 mm lateral field of view and a 100 μm axial depth of field. We demonstrate our technique on healthy and hypertensive, freshly excised and intact mouse aortas. Our technique has the capacity to delineate the individual mechanics of elastic lamellae and vascular smooth muscle. Further, we observe global and regional vascular stiffening in hypertensive aortas, and note the presence of local micro-mechanical signatures, characteristic of fibrous and lipid-rich regions.

Original language	English
Title of host publication	International Conference on Biophotonics V
Editors	David D. Sampson, Dennis L. Matthews, Jurgen Popp, Halina Rubinsztein-Dunlop, Brian C. Wilson
Publisher	SPIE
Number of pages	1
ISBN (Electronic)	9781510611269
ISBN (Print)	9781510611252
DOIs	https://doi.org/10.1117/12.2269903
Publication status	Published - 29 Apr 2017
Event	5th International Conference on Biophotonics, ICOB 2017 - Perth, Australia Duration: 30 Apr 2017 → 1 May 2017 Conference number: 5 http://www.icob2017.com

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Publisher	SPIE
Volume	10340
ISSN (Print)	1605-7422
ISSN (Electronic)	2410-9045

Conference

Conference	5th International Conference on Biophotonics, ICOB 2017
Abbreviated title	ICOB 2017
Country/Territory	Australia
City	Perth
Period	30/04/17 → 1/05/17
Internet address	http://www.icob2017.com

Access to Document

10.1117/12.2269903

Wijesinghe-2017-Optical-coherence-SPIE-1034010
Copyright © 2017 SPIE. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1117/12.2269903
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Wijesinghe, P., Johansen, N. J., Curatolo, A., Sampson, D. D., Ganss, R., & Kennedy, B. F. (2017). Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta. In D. D. Sampson, D. L. Matthews, J. Popp, H. Rubinsztein-Dunlop, & B. C. Wilson (Eds.), International Conference on Biophotonics V Article 1034010 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10340). SPIE. https://doi.org/10.1117/12.2269903

Wijesinghe, Philip ; Johansen, Niloufer J. ; Curatolo, Andrea et al. / Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta. International Conference on Biophotonics V. editor / David D. Sampson ; Dennis L. Matthews ; Jurgen Popp ; Halina Rubinsztein-Dunlop ; Brian C. Wilson. SPIE, 2017. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta",

abstract = "We have developed a high-resolution optical coherence elastography system capable of estimating Young's modulus in tissue volumes with an isotropic resolution of 15 μm over a 1 mm lateral field of view and a 100 μm axial depth of field. We demonstrate our technique on healthy and hypertensive, freshly excised and intact mouse aortas. Our technique has the capacity to delineate the individual mechanics of elastic lamellae and vascular smooth muscle. Further, we observe global and regional vascular stiffening in hypertensive aortas, and note the presence of local micro-mechanical signatures, characteristic of fibrous and lipid-rich regions.",

author = "Philip Wijesinghe and Johansen, {Niloufer J.} and Andrea Curatolo and Sampson, {David D.} and Ruth Ganss and Kennedy, {Brendan F.}",

year = "2017",

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booktitle = "International Conference on Biophotonics V",

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Wijesinghe, P, Johansen, NJ, Curatolo, A, Sampson, DD, Ganss, R & Kennedy, BF 2017, Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta. in DD Sampson, DL Matthews, J Popp, H Rubinsztein-Dunlop & BC Wilson (eds), International Conference on Biophotonics V., 1034010, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10340, SPIE, 5th International Conference on Biophotonics, ICOB 2017, Perth, Western Australia, Australia, 30/04/17. https://doi.org/10.1117/12.2269903

Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta. / Wijesinghe, Philip; Johansen, Niloufer J.; Curatolo, Andrea et al.
International Conference on Biophotonics V. ed. / David D. Sampson; Dennis L. Matthews; Jurgen Popp; Halina Rubinsztein-Dunlop; Brian C. Wilson. SPIE, 2017. 1034010 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10340).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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T1 - Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta

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AU - Johansen, Niloufer J.

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AU - Kennedy, Brendan F.

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AB - We have developed a high-resolution optical coherence elastography system capable of estimating Young's modulus in tissue volumes with an isotropic resolution of 15 μm over a 1 mm lateral field of view and a 100 μm axial depth of field. We demonstrate our technique on healthy and hypertensive, freshly excised and intact mouse aortas. Our technique has the capacity to delineate the individual mechanics of elastic lamellae and vascular smooth muscle. Further, we observe global and regional vascular stiffening in hypertensive aortas, and note the presence of local micro-mechanical signatures, characteristic of fibrous and lipid-rich regions.

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DO - 10.1117/12.2269903

M3 - Conference contribution

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SN - 9781510611252

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Wijesinghe P, Johansen NJ, Curatolo A, Sampson DD, Ganss R, Kennedy BF. Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta. In Sampson DD, Matthews DL, Popp J, Rubinsztein-Dunlop H, Wilson BC, editors, International Conference on Biophotonics V. SPIE. 2017. 1034010. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2269903

Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta

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