Optical chromatography using a photonic crystal fiber with on-chip fluorescence excitation

P. C. Ashok, R. F. Marchington, P. Mthunzi, T. F. Krauss, K. Dholakia

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


We describe the realization of integrated optical chromatography, in conjunction with on-chip fluorescence excitation, in a monolithically fabricated poly-dimethylsiloxane (PDMS) microfluidic chip. The unique endlessly-single-mode guiding property of the Photonic Crystal Fiber (PCF) facilitates simultaneous on-chip delivery of beams to perform optical sorting in conjunction with fluorescence excitation. We use soft lithography to define the chip and insert the specially capped PCF into it through a predefined fiber channel that is intrinsically aligned with the sorting channel. We compare the performance of the system to a standard ray optics model and use the system to demonstrate both size-driven and refractive index-driven separations of colloids. Finally we demonstrate a new technique of enhanced optofluidic separation of biological particles, by sorting of human kidney embryonic cells (HEK-293), internally tagged with fluorescing microspheres through phagocytocis, from those without microspheres and the separation purity is monitored using fluorescence imaging. (C) 2010 Optical Society of America

Original languageEnglish
Pages (from-to)6396-6407
Number of pages12
JournalOptics Express
Issue number6
Early online date12 Mar 2010
Publication statusPublished - 15 Mar 2010


  • Optical sorting
  • Microfluidics
  • Photonic crystal fiber
  • optical manipulation


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