Onchorhyncin III: a potent antimicrobial peptide derived from the non-histone chromosomal protein H6 of rainbow trout, Oncorhynchus mykiss

Jorge Fernandes, N Saint, Graham Duncan Kemp, Valerie Jane Smith

Research output: Contribution to journalArticlepeer-review

80 Citations (Scopus)

Abstract

A 6.7 kDa antimicrobial peptide was isolated from trout skin secretions using acid extraction followed by cation-exchange chromatography, C-18, solid-phase extraction, and C-18 reversed-phase HPLC. The molecular mass of this peptide, which is tentatively named oncorhyncin III, is 6671 Da, as determined by matrix-assisted laser-desorption ionization MS. N-terminal amino acid sequencing revealed that the first 13 residues of oncorhyncin III are identical with those of the non-histone chromosomal protein H6 from rainbow trout. Hence these data combined with the MS results indicate that oncorhyncin III is likely to be a cleavage product of the non-histone chromosomal protein H6 (residues 1-66) and that it probably contains two methylated residues or one double methylation. The purified peptide exhibits potent antibacterial activity against both Gram-positive and Gram-negative bacteria, with minimal inhibitory concentrations in the submicromolar range. The peptide is sensitive to NaCl, and displays no haemolytic activity towards trout erythrocytes at concentrations below 1 muM. Scanning electron microscopy revealed that oncorhyncin III does not cause direct disruption of bacterial cells. Reconstitution of the peptide in planar lipid bilayers strongly disturbs the membranes, but does not induce the formation of stable ion channels. Taken together, these results support the hypothesis that oncorhyncin III plays a role in mucosal innate host defence.

Original languageEnglish
Pages (from-to)621-628
Number of pages8
JournalBiochemical Journal
Volume373
Issue number2
DOIs
Publication statusPublished - 15 Jul 2003

Keywords

  • antimicrobial peptide
  • historic
  • mucosal immunity
  • oncorhyncin III
  • teleost
  • trout
  • HUMAN CDNA SEQUENCE
  • HISTONE H2A
  • MULTIGENE FAMILY
  • SKIN SECRETIONS
  • ATLANTIC SALMON
  • MECHANISM
  • MEMBRANE
  • BINDING
  • HMG-14
  • TESTIS

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