Okadaic acid induces Golgi apparatus fragmentation and arrest of intracellular transport

J Lucocq, G Warren, J Pryde

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106 Citations (Scopus)


The specific phosphatase inhibitor okadaic acid (OA) induced fragmentation of the Golgi apparatus in interphase HeLa cells. Immunoelectron microscopy for galactosyltransferase identified a major Golgi fragment composed of a cluster of vesicles and tubules that was morphologically indistinguishable from the 'Golgi cluster' previously described in mitotic cells. The presence of homogeneous immunofluorescence staining for galactosyltransferase in OA-treated cells also suggested that isolated Golgi vesicles, previously found in mitotic cells, existed along with the clusters. After removal of OA, both clusters and vesicles appeared to participate in a reassembly pathway that strongly resembled that occurring during telophase. OA also induced inhibition of intracellular transport, another feature of mitotic cells. OA treatment prevented newly synthesised G protein of vesicular stomatitis virus (VSV) from acquiring resistance to endoglycosidase H and from arriving at the cell surface. In addition, fluid phase endocytosis of horseradish peroxidase (HRP) was reduced to less than 10% of control values. All these effects were dose-dependent and reversible. OA should be a useful tool to study the Golgi division and membrane traffic.
Original languageEnglish
Pages (from-to)753-9
Number of pages7
JournalJournal of Cell Science
Volume100 ( Pt 4)
Publication statusPublished - Dec 1991


  • Biological Transport
  • Endocytosis
  • Ethers, Cyclic
  • Galactosyltransferases
  • Golgi Apparatus
  • HeLa Cells
  • Horseradish Peroxidase
  • Humans
  • Interphase
  • Ionophores
  • Membrane Glycoproteins
  • Microscopy, Fluorescence
  • Morphogenesis
  • Okadaic Acid
  • Phosphoprotein Phosphatases
  • Viral Envelope Proteins


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