Occurrence, prevalence and viral load of deformed wing virus variants in Apis mellifera colonies in Chile

Gustavo Riveros; Nolberto Arismendi; Nelson Zapata; David Evans; Ivonne Pérez; Patricia Aldea; Marisol Vargas

doi:10.1080/00218839.2019.1670993

Occurrence, prevalence and viral load of deformed wing virus variants in Apis mellifera colonies in Chile

Gustavo Riveros, Nolberto Arismendi, Nelson Zapata, David Evans, Ivonne Pérez, Patricia Aldea, Marisol Vargas

Research output: Contribution to journal › Article › peer-review

Abstract

Deformed wing virus (DWV) is one of the most common viruses in apiaries worldwide, and its presence in Chilean apiaries is no exception. There are three well-defined master variants described as DWV-A, DWV-B (or VDV-1) and DWV-C. We studied the prevalence, load and recombinant genotypes among DWV variants in honey bees from Chilean apiaries. We also compared the viral load in each region in colonies that were treated or untreated against Varroa destructor. Using real-time PCR with specific primers enabled us to determine that DWV-A was the most prevalent (71%) throughout Chile, with a higher level than DWV-B (circa 3% prevalence), and almost 60-times more load, especially in northern Chile. The viral load was lower only in treated colonies located in the Metropolitana, Biobío/Ñuble and Los Ríos regions. The DWV-C genotype and recombinants were not detected. This suggests that viral recombinant events are limited since DWV-B is still uncommon in honey bee hives at present. This is the first study involving DWV-B in Chile.

Original language	English
Pages (from-to)	63-68
Number of pages	6
Journal	Journal of Apicultural Research
Volume	59
Issue number	1
Early online date	9 Oct 2019
DOIs	https://doi.org/10.1080/00218839.2019.1670993
Publication status	Published - 2020

Keywords

Honey bees
DWV variants
DWV-B
DWV-A
Viral load
Real-time PCR

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Riveros-2019-Occurence-prevalence-JAR-AAM
Copyright © 2019 International Bee Research Association. This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1080/00218839.2019.1670993
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Cite this

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title = "Occurrence, prevalence and viral load of deformed wing virus variants in Apis mellifera colonies in Chile",

abstract = "Deformed wing virus (DWV) is one of the most common viruses in apiaries worldwide, and its presence in Chilean apiaries is no exception. There are three well-defined master variants described as DWV-A, DWV-B (or VDV-1) and DWV-C. We studied the prevalence, load and recombinant genotypes among DWV variants in honey bees from Chilean apiaries. We also compared the viral load in each region in colonies that were treated or untreated against Varroa destructor. Using real-time PCR with specific primers enabled us to determine that DWV-A was the most prevalent (71%) throughout Chile, with a higher level than DWV-B (circa 3% prevalence), and almost 60-times more load, especially in northern Chile. The viral load was lower only in treated colonies located in the Metropolitana, Biob{\'i}o/{\~N}uble and Los R{\'i}os regions. The DWV-C genotype and recombinants were not detected. This suggests that viral recombinant events are limited since DWV-B is still uncommon in honey bee hives at present. This is the first study involving DWV-B in Chile.",

keywords = "Honey bees, DWV variants, DWV-B, DWV-A, Viral load, Real-time PCR",

author = "Gustavo Riveros and Nolberto Arismendi and Nelson Zapata and David Evans and Ivonne P{\'e}rez and Patricia Aldea and Marisol Vargas",

note = "This study was supported by FONDECYT Grant N° 1171781 from the National Commission for Scientific and Technological Research, CONICYT, Chile.",

year = "2020",

doi = "10.1080/00218839.2019.1670993",

language = "English",

volume = "59",

pages = "63--68",

journal = "Journal of Apicultural Research",

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T1 - Occurrence, prevalence and viral load of deformed wing virus variants in Apis mellifera colonies in Chile

AU - Riveros, Gustavo

AU - Arismendi, Nolberto

AU - Zapata, Nelson

AU - Evans, David

AU - Pérez, Ivonne

AU - Aldea, Patricia

AU - Vargas, Marisol

N1 - This study was supported by FONDECYT Grant N° 1171781 from the National Commission for Scientific and Technological Research, CONICYT, Chile.

PY - 2020

Y1 - 2020

N2 - Deformed wing virus (DWV) is one of the most common viruses in apiaries worldwide, and its presence in Chilean apiaries is no exception. There are three well-defined master variants described as DWV-A, DWV-B (or VDV-1) and DWV-C. We studied the prevalence, load and recombinant genotypes among DWV variants in honey bees from Chilean apiaries. We also compared the viral load in each region in colonies that were treated or untreated against Varroa destructor. Using real-time PCR with specific primers enabled us to determine that DWV-A was the most prevalent (71%) throughout Chile, with a higher level than DWV-B (circa 3% prevalence), and almost 60-times more load, especially in northern Chile. The viral load was lower only in treated colonies located in the Metropolitana, Biobío/Ñuble and Los Ríos regions. The DWV-C genotype and recombinants were not detected. This suggests that viral recombinant events are limited since DWV-B is still uncommon in honey bee hives at present. This is the first study involving DWV-B in Chile.

AB - Deformed wing virus (DWV) is one of the most common viruses in apiaries worldwide, and its presence in Chilean apiaries is no exception. There are three well-defined master variants described as DWV-A, DWV-B (or VDV-1) and DWV-C. We studied the prevalence, load and recombinant genotypes among DWV variants in honey bees from Chilean apiaries. We also compared the viral load in each region in colonies that were treated or untreated against Varroa destructor. Using real-time PCR with specific primers enabled us to determine that DWV-A was the most prevalent (71%) throughout Chile, with a higher level than DWV-B (circa 3% prevalence), and almost 60-times more load, especially in northern Chile. The viral load was lower only in treated colonies located in the Metropolitana, Biobío/Ñuble and Los Ríos regions. The DWV-C genotype and recombinants were not detected. This suggests that viral recombinant events are limited since DWV-B is still uncommon in honey bee hives at present. This is the first study involving DWV-B in Chile.

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KW - DWV variants

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KW - DWV-A

KW - Viral load

KW - Real-time PCR

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DO - 10.1080/00218839.2019.1670993

M3 - Article

SN - 0021-8839

VL - 59

SP - 63

EP - 68

JO - Journal of Apicultural Research

JF - Journal of Apicultural Research

IS - 1

ER -

Occurrence, prevalence and viral load of deformed wing virus variants in Apis mellifera colonies in Chile

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Keywords

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