Nucleosome mobilization catalysed by the yeast SWI/SNF complex

I Whitehouse, A Flaus, BR Cairns, Malcolm Frederick White, JL Workman, T Owen-Hughes

Research output: Contribution to journalArticlepeer-review

277 Citations (Scopus)

Abstract

The generation of a local chromatin topology conducive to transcription is a key step in gene regulation(1). The yeast SWI/SNF complex is the founding member of a family of ATP-dependent remodelling activities capable of altering chromatin structure both in vitro and in vivo(2). Despite its importance, the pathway by which the SWI/SNF complex disrupts chromatin structure is unknown. Here we use a model system to demonstrate that the yeast SWI/SNF complex can reposition nucleosomes in an ATP-dependent reaction that favours attachment of the histone octamer to an acceptor site on the same molecule of DNA (in cis), We show that SWI/SNF-mediated displacement of the histone octamer is effectively blocked by a barrier introduced into the DNA, suggesting that this redistribution involves sliding or tracking of nucleosomes along DNA, and that it is achieved by a catalytic mechanism. We conclude that SWI/SNF catalyses the redistribution of nucleosomes along DNA in cis, which may represent a general mechanism by which ATP-dependent chromatin remodelling occurs.

Original languageEnglish
Pages (from-to)784-787
Number of pages4
JournalNature
Volume400
Issue number6746
Publication statusPublished - 19 Aug 1999

Keywords

  • REMODELING FACTOR
  • HOLLIDAY JUNCTION
  • MOBILITY
  • BINDING
  • TRANSCRIPTION
  • RESOLUTION
  • ISWI
  • RSC
  • DNA

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