Nucleic acid sequence relationships between enterovirus serotypes

Timo Hyypiä*, Marita Maaronen, Petri Auvinen, Per Stålhandske, Ulf Pettersson, Glyn Stanway, Pamela Hughes, Martin Ryan, Jeffrey Almond, Mirja Stenvik, Tapani Hovi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Forty-eight different enterovirus serotypes were analysed by a nucleic-acid hybridization test using probes derived from the 3′ end of coxsackievirus A21 (CA21) and B3 (CB3), poliovirus 3 (P3) and enterovirus 70 (E70). More than 90% of the serotypes could be detected with this collection of reagents. The CB3 probe reacted with all the coxsackie B viruses, with all three poliovirus serotypes, and with almost all of the 30 ECHO virus types tested. In addition some of the coxsackie A viruses and the BrDr 73 strain of enterovirus 71 gave a positive signal with this probe. The P3 probe detected all the poliovirus strains and also some coxsackievirus A isolates but no coxsackie B or ECHO viruses. A similar hybridization pattern as with the P3 probe was obtained when the CA 21 probe was used. The E70 probe appeared to be strain-specific. The results indicate that nucleic-acid hybridization is a useful method for rapid detection and subgrouping of enteroviruses during virus isolation, and that the test could be further developed for typing of the strains.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalMolecular and Cellular Probes
Volume1
Issue number2
DOIs
Publication statusPublished - 1 Jan 1987

Keywords

  • enteroviruses
  • nucleic-acid hybridization

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