TY - JOUR
T1 - Nuclear and plastid SNP markers for tracing Cedrela timber in the tropics
AU - Paredes-Villanueva, Kathelyn
AU - Blanc-Jolivet, Celine
AU - Mader, Malte
AU - Honorio Coronado, Eurídice N.
AU - Garcia-Davila, Carmen
AU - Sebbenn, Alexandre M.
AU - Rocha Venancio Meyer-Sand, Barbara
AU - Caron, Henri
AU - Tysklind, Niklas
AU - Cavers, Stephen
AU - Degen, Bernd
N1 - Funding Information:
This research was supported by the German Federal Ministry of Food and Agriculture in the Frame of the “Large scale project on genetic timber verification”. Genotyping was performed at the Genomic and Sequencing Facility of Bordeaux (Grants from the Conseil Regional d’Aquitaine n° 20030304002FA and 20040305003FA, the European Union, FEDER N° 2003227, and Investissements d’avenir, n° ANR-10-EQPX-16-01 and CEBA: ANR-10-LABX-25-01). Permits ICMBIO N° 47960 and 49369, Registration SisGen N° A16E4DA (Brazil), MMAYA/VMABCCGDF/DGBAP/MEG N° 0280/2016 (Bolivia), R.D. N° 001-2016-SERNANP-DGANP, R.D. N° 001A-2015-SERFOR-DGGSPFFS-DGSPF, Contrato N° 001-2016-SERFOR-DGGSPFFS-DGSPF (Peru) were granted for collection, transport and genetic analyses.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Illegal logging and trade of timber are major worldwide concerns, resulting in biodiversity and economic losses. Tropical tree species in the genus Cedrela, which have historically been heavily exploited, are still often illegally traded and there is an urgent need to develop tools to verify the origin of Cedrela products. A set of 351 SNP loci for Cedrela species from Bolivia, Brazil, Costa Rica, Cuba, Ecuador, French Guiana, Mexico, and Peru was developed using restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing, and adapted for MassARRAY genotyping. After screening of 94 individuals covering most of the distribution of Cedrela, a final set of 136 SNP loci which included 92 nuclear SNPs, 22 chloroplast markers (20 SNPs and 2 INDELs), and 22 mitochondrial markers (19 SNPs and 3 INDELs) was selected and tested for potential to verify Cedrela timber origin.
AB - Illegal logging and trade of timber are major worldwide concerns, resulting in biodiversity and economic losses. Tropical tree species in the genus Cedrela, which have historically been heavily exploited, are still often illegally traded and there is an urgent need to develop tools to verify the origin of Cedrela products. A set of 351 SNP loci for Cedrela species from Bolivia, Brazil, Costa Rica, Cuba, Ecuador, French Guiana, Mexico, and Peru was developed using restriction associated DNA sequencing (RADSeq) and low coverage MiSeq genome sequencing, and adapted for MassARRAY genotyping. After screening of 94 individuals covering most of the distribution of Cedrela, a final set of 136 SNP loci which included 92 nuclear SNPs, 22 chloroplast markers (20 SNPs and 2 INDELs), and 22 mitochondrial markers (19 SNPs and 3 INDELs) was selected and tested for potential to verify Cedrela timber origin.
KW - Cedrela
KW - MassARRAY
KW - MiSeq
KW - RADSeq
KW - SNP
KW - Tracing origin
KW - Tropical timber
U2 - 10.1007/s12686-019-01110-1
DO - 10.1007/s12686-019-01110-1
M3 - Letter
AN - SCOPUS:85070858081
SN - 1877-7252
VL - 12
SP - 239
EP - 244
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -