Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species

S. O. Regeai; K. M. Dolan; D. A. Fitzpatrick; J. A. Browne; J. T. Jones; A. M. Burnell

doi:10.1111/j.1755-0998.2008.02431.x

Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species

S. O. Regeai, K. M. Dolan, D. A. Fitzpatrick, J. A. Browne, J. T. Jones, A. M. Burnell

Research output: Contribution to journal › Article › peer-review

Abstract

We describe 24 novel primers that amplify intron regions in housekeeping and structural genes of Heterorhabditis bacteriophora. The cross-amplification potential of these primers in seven other Heterorhabditis species was determined. The results obtained showed interspecific nucleotide, length and splice site variability in the sequenced introns and for one gene, an intron gain was observed. These primers will be useful tools for studying population genetics, genetic diversity and intron DNA evolution within the genus Heterorhabditis and other genera of rhabditid nematodes.

Original language	English
Pages (from-to)	421-424
Number of pages	4
Journal	Molecular Ecology Resources
Volume	9
Issue number	1
DOIs	https://doi.org/10.1111/j.1755-0998.2008.02431.x
Publication status	Published - Jan 2009

Keywords

Entomopathogenic
EPIC-PCR
Heterorhabditis
Intron
Molecular markers
Nematode

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10.1111/j.1755-0998.2008.02431.x

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Regeai, S. O., Dolan, K. M., Fitzpatrick, D. A., Browne, J. A., Jones, J. T., & Burnell, A. M. (2009). Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species. Molecular Ecology Resources, 9(1), 421-424. https://doi.org/10.1111/j.1755-0998.2008.02431.x

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abstract = "We describe 24 novel primers that amplify intron regions in housekeeping and structural genes of Heterorhabditis bacteriophora. The cross-amplification potential of these primers in seven other Heterorhabditis species was determined. The results obtained showed interspecific nucleotide, length and splice site variability in the sequenced introns and for one gene, an intron gain was observed. These primers will be useful tools for studying population genetics, genetic diversity and intron DNA evolution within the genus Heterorhabditis and other genera of rhabditid nematodes.",

keywords = "Entomopathogenic, EPIC-PCR, Heterorhabditis, Intron, Molecular markers, Nematode",

author = "Regeai, {S. O.} and Dolan, {K. M.} and Fitzpatrick, {D. A.} and Browne, {J. A.} and Jones, {J. T.} and Burnell, {A. M.}",

year = "2009",

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doi = "10.1111/j.1755-0998.2008.02431.x",

language = "English",

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Regeai, SO, Dolan, KM, Fitzpatrick, DA, Browne, JA, Jones, JT & Burnell, AM 2009, 'Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species', Molecular Ecology Resources, vol. 9, no. 1, pp. 421-424. https://doi.org/10.1111/j.1755-0998.2008.02431.x

Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species. / Regeai, S. O.; Dolan, K. M.; Fitzpatrick, D. A. et al.
In: Molecular Ecology Resources, Vol. 9, No. 1, 01.2009, p. 421-424.

Research output: Contribution to journal › Article › peer-review

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T1 - Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species

AU - Regeai, S. O.

AU - Dolan, K. M.

AU - Fitzpatrick, D. A.

AU - Browne, J. A.

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AU - Burnell, A. M.

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KW - Entomopathogenic

KW - EPIC-PCR

KW - Heterorhabditis

KW - Intron

KW - Molecular markers

KW - Nematode

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VL - 9

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Novel primers for the amplification of nuclear DNA introns in the entomopathogenic nematode Heterorhabditis bacteriophora and their cross-amplification in seven other Heterorhabditis species

Abstract

Keywords

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