Noradrenergic modulation of XII motoneuron inspiratory activity does not involve {alpha}2 receptor inhibition of the Ih current or presynaptic glutamate release.

T Adachi, D M Robinson, Gareth Brian Miles, G D Funk

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Norepinephrine has powerful and diverse modulatory effects on hypoglossal (XII) motoneuron activity, which is important in maintaining airway patency. The objective was to test two hypotheses that alpha(2)-adrenoceptor-mediated, presynaptic inhibition of glutamatergic inspiratory drive ( Selvaratnam SR, Parkis MA, and Funk GD. Brain Res 805: 104 - 115, 1998) and postsynaptic inhibition of the hyperpolarization- activated inward current (I-h) ( Parkis MA and Berger AJ. Brain Res 769: 108 - 118, 1997) modulate XII inspiratory activity. Nerve and whole cell recordings were applied to rhythmic medullary slice preparations from neonatal rats ( postnatal days 0 - 4) to monitor XII inspiratory burst amplitude and motoneuron properties. Application of an alpha(2)-receptor agonist ( clonidine, 1 mM) to the XII nucleus reduced inspiratory burst amplitude to 71 +/- 3% of control but had no effect on inspiratory synaptic currents. It also reduced the Ih current by similar to 40%, but an I-h current blocker ( ZD7288), at concentrations that blocked similar to 80% of I-h, had no effect on inspiratory burst amplitude. The clonidine inhibition was unaffected by the GABA(A) antagonist (+) bicuculline but attenuated by the alpha(2)-antagonist rauwolscine and the imidazoline 1 ( I-1) antagonist efaroxan. The I-1 agonist rilmenidine, but not the alpha(2)-agonist UK14304, inhibited XII output. Clonidine also reduced action potential amplitude or impaired repetitive firing. Although a contribution from alpha(2), and in particular I-1, receptors remains possible, results demonstrate that 1) noradrenergic modulation of XII inspiratory activity is unlikely to involve alpha(2)-receptor- mediated presynaptic inhibition of glutamate release or modulation of Ih; 2) inhibition of repetitive firing is a major factor underlying the inhibition of XII output by clonidine; and 3) Ih is present in neonatal XII motoneurons but does not contribute to shaping their inspiratory activity.

Original languageEnglish
Pages (from-to)1297-1308
Number of pages12
JournalJournal of Applied Physiology
Volume98
Issue number4
DOIs
Publication statusPublished - Apr 2005

Keywords

  • airway control
  • alpha(2)-adrenoceptor
  • hyperpolarization-activated inward current
  • whole cell recording
  • rat
  • clonidine
  • HYPERPOLARIZATION-ACTIVATED CURRENT
  • RAT HYPOGLOSSAL MOTONEURONS
  • SYMPATHETIC PREGANGLIONIC NEURONS
  • RECEPTOR-LIKE IMMUNOREACTIVITY
  • CENTRAL-NERVOUS-SYSTEM
  • INFANT-DEATH-SYNDROME
  • RESPIRATORY RHYTHM
  • ALPHA(2)-ADRENERGIC RECEPTORS
  • SYNAPTIC-TRANSMISSION
  • INWARD CURRENT

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