Non-centrosomal microtubule-organising centres in cold-treated cultured Drosophila cells

Deborah M. Cottam, John B. Tucker, Margaret M. Rogers-Bald, John B. Mackie, John Macintyre, Julie A. Scarborough, Hiroyuki Ohkura, Martin John Milner

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

In this paper we describe a new type of non-centrosomal microtubule-organising centre (MTOC), which is induced by cold treatment of certain cultured Drosohhila cells and allows rapid reassembly of microtubule (MT) arrays. Prolonged cooling of two types of cultured Drosophila cells, muscle cells in primary culture and a wing imaginal disc cell line C1.8+ results in disassembly of MT arrays and induces the formation of clusters of short MTs that have not been,described before. Upon rewarming, the clusters are lost and the MT array is re-established within 1 h. In C1.8+ cells, gamma-tubulin-containing centrosomes are detected, both in cell extensions and in the expected juxtanuclear position, and gamma-tubulin co-localises with the cold-induced MT clusters. The MT plus-end-binding protein, Drosophila EB1, decorates growing tips of MTs extending from clusters. We conclude that the cold-induced MT clusters represent acentrosomal MTOCs, allowing rapid reassembly of MT arrays following exposure to cold.

Original languageEnglish
Pages (from-to)88-100
Number of pages13
JournalCell Motility and the Cytoskeleton
Volume63
DOIs
Publication statusPublished - Feb 2006

Keywords

  • acentrosomal
  • gamma-tubulin
  • muscle
  • cell culture
  • Clone 8
  • primary embryonic culture
  • GAMMA-TUBULIN
  • DIFFERENTIATION INVITRO
  • CAENORHABDITIS-ELEGANS
  • MITOTIC SPINDLE
  • EMBRYONIC CELLS
  • IMAGINAL DISKS
  • NUCLEATION
  • EB1
  • DYNAMICS
  • ORGANIZATION

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