Abstract
New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.
| Original language | English |
|---|---|
| Pages (from-to) | 252-4 |
| Number of pages | 3 |
| Journal | Journal of Clinical Microbiology |
| Volume | 45 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2007 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- Antibiotics, Antitubercular/pharmacology
- Bacterial Proteins/genetics
- DNA Primers
- DNA-Directed RNA Polymerases
- Drug Resistance, Bacterial/genetics
- Humans
- Mycobacterium tuberculosis/drug effects
- Polymerase Chain Reaction/methods
- Rifampin/pharmacology
- Sensitivity and Specificity
- Sputum/microbiology
- Tuberculosis, Pulmonary/microbiology
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