Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts; Oscar Nolasco; Pim de Rijk; Elie Nduwamahoro; Armand Van Deun; Andy Ramsay; Jorge Arevalo; Françoise Portaels

doi:10.1128/JCM.01489-06

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Leen Rigouts, Oscar Nolasco, Pim de Rijk, Elie Nduwamahoro, Armand Van Deun, Andy Ramsay, Jorge Arevalo, Françoise Portaels

Research output: Contribution to journal › Article › peer-review

Abstract

New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

Original language	English
Pages (from-to)	252-4
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	1
DOIs	https://doi.org/10.1128/JCM.01489-06
Publication status	Published - Jan 2007

Keywords

Antibiotics, Antitubercular/pharmacology
Bacterial Proteins/genetics
DNA Primers
DNA-Directed RNA Polymerases
Drug Resistance, Bacterial/genetics
Humans
Mycobacterium tuberculosis/drug effects
Polymerase Chain Reaction/methods
Rifampin/pharmacology
Sensitivity and Specificity
Sputum/microbiology
Tuberculosis, Pulmonary/microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/JCM.01489-06

Cite this

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title = "Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis",

abstract = "New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.",

keywords = "Antibiotics, Antitubercular/pharmacology, Bacterial Proteins/genetics, DNA Primers, DNA-Directed RNA Polymerases, Drug Resistance, Bacterial/genetics, Humans, Mycobacterium tuberculosis/drug effects, Polymerase Chain Reaction/methods, Rifampin/pharmacology, Sensitivity and Specificity, Sputum/microbiology, Tuberculosis, Pulmonary/microbiology",

author = "Leen Rigouts and Oscar Nolasco and {de Rijk}, Pim and Elie Nduwamahoro and {Van Deun}, Armand and Andy Ramsay and Jorge Arevalo and Fran{\c c}oise Portaels",

year = "2007",

month = jan,

doi = "10.1128/JCM.01489-06",

language = "English",

volume = "45",

pages = "252--4",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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T1 - Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

AU - Rigouts, Leen

AU - Nolasco, Oscar

AU - de Rijk, Pim

AU - Nduwamahoro, Elie

AU - Van Deun, Armand

AU - Ramsay, Andy

AU - Arevalo, Jorge

AU - Portaels, Françoise

PY - 2007/1

Y1 - 2007/1

N2 - New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

AB - New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

KW - Antibiotics, Antitubercular/pharmacology

KW - Bacterial Proteins/genetics

KW - DNA Primers

KW - DNA-Directed RNA Polymerases

KW - Drug Resistance, Bacterial/genetics

KW - Humans

KW - Mycobacterium tuberculosis/drug effects

KW - Polymerase Chain Reaction/methods

KW - Rifampin/pharmacology

KW - Sensitivity and Specificity

KW - Sputum/microbiology

KW - Tuberculosis, Pulmonary/microbiology

U2 - 10.1128/JCM.01489-06

DO - 10.1128/JCM.01489-06

M3 - Article

C2 - 17093024

SN - 0095-1137

VL - 45

SP - 252

EP - 254

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 1

ER -

Newly developed primers for comprehensive amplification of the rpoB gene and detection of rifampin resistance in Mycobacterium tuberculosis

Abstract

Keywords

UN SDGs

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