Naturally occurring tyrosine kinase inserts block high affinity binding of phospholipase Cg and Shc to TrkC, and neurotrophin-3 signalling.

M Guiton, Francis James Gunn-Moore, DJ Glass, DR Geis, G Yancopolous, JM Tavare

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Neurotrophin-3 binds to the receptor tyrosine kinase, TrkC. Several naturally occurring splice variants of TrkC exist including those with 14- and 39 amino acid inserts within the tyrosine kinase homology region. When expressed in fibroblasts, full-length TrkC, but not the kinase insert variants, mediated neurotrophin-3-stimulated cell proliferation. We investigated the molecular basis of this signaling defect. The kinase inserts blocked the ability of TrkC to mediate neurotrophin-3 stimulated c-myc and c-fos transcription and activation of the AP-1 transcriptional complex. In cells expressing full-length TrkC, neurotrophin-3 promoted a sustained activation of mitogen-activated protein kinase; TrkC containing kinase inserts only mediated transient activation of mitogen-activated protein kinase. The kinase inserts specifically blocked neurotrophin-3-stimulated autophosphorylation of the phospholipase C gamma binding site on TrkC (tyrosine 789) resulting in a severe reduction in phospholipase C gamma association with TrkC and its tyrosine phosphorylation. Neurotrophin-3-stimulated phosphorylation of the She binding site (tyrosine 485) on TrkC, and tyrosine phosphorylation of She itself, was unaffected by the kinase inserts; however, the kinase inserts blocked high affinity She association with TrkC. It is proposed that the lack of high affinity binding of Shc and/or phospholipase C gamma to the TrkC kinase insert variants may be responsible for the inability of these variants to bring about a full biological response in fibroblasts.

Original languageEnglish
Pages (from-to)20384-20390
Number of pages7
JournalJournal of Biological Chemistry
Volume270
Issue number35
DOIs
Publication statusPublished - 1 Sept 1995

Keywords

  • NERVE GROWTH-FACTOR
  • INSULIN-RECEPTOR
  • PROTEIN-KINASE
  • RAT TRKC
  • CELLS
  • PHOSPHORYLATION
  • ACTIVATION
  • ENCODES
  • AUTOPHOSPHORYLATION
  • IDENTIFICATION

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