Narrow stimulated resonance Raman scattering and WGM lasing in small conjugated polymer particles for live cell tagging and tracking

Bastian Haehnle; Markus Lamla,; Konstantin M. J Sparrer,; Malte C. Gather; Alexander J. C. Kuehne

doi:10.1002/adom.202001553

Narrow stimulated resonance Raman scattering and WGM lasing in small conjugated polymer particles for live cell tagging and tracking

Bastian Haehnle, Markus Lamla,, Konstantin M. J Sparrer,, Malte C. Gather, Alexander J. C. Kuehne

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Abstract

Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here we present, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles as a new method for generating narrow emission as an alternative to WGM-based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. We discuss the synthesis of monodisperse micron-sized poly(fluorene-co-divinylbenzene) particles and
characterize their WGM and SRRS emission. Finally, we showcase how these particles and their emission can be employed in live cell imaging and tagging.

Original language	English
Article number	2001553
Journal	Advanced Optical Materials
Volume	Early View
Early online date	9 Dec 2020
DOIs	https://doi.org/10.1002/adom.202001553
Publication status	E-pub ahead of print - 9 Dec 2020

Keywords

live cell imaging
Conjugated polymer particles
Laser emission
Whispering gallery mode

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10.1002/adom.202001553Licence: CC BY-NC

Haehnle_2020_AOM_Narrow_CC
Copyright © 2020 The Authors. Advanced Optical Materials published by Wiley‐VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Final published version, 2.21 MBLicence: CC BY-NC

H2020 ERC Starting Grant ABLASE: H2020 ERC Starting Grant ABLASE (Grant Agreement No. 640012)
Gather, M. C. (PI)
European Research Council
1/06/15 → 31/05/21
Project: Standard

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@article{64ae30d7ae1a4eada44bd867c2ab7fc1,

title = "Narrow stimulated resonance Raman scattering and WGM lasing in small conjugated polymer particles for live cell tagging and tracking",

abstract = "Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here we present, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles as a new method for generating narrow emission as an alternative to WGM-based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. We discuss the synthesis of monodisperse micron-sized poly(fluorene-co-divinylbenzene) particles andcharacterize their WGM and SRRS emission. Finally, we showcase how these particles and their emission can be employed in live cell imaging and tagging.",

keywords = "live cell imaging, Conjugated polymer particles, Laser emission, Whispering gallery mode",

author = "Bastian Haehnle and Markus Lamla, and Sparrer,, {Konstantin M. J} and Gather, {Malte C.} and Kuehne, {Alexander J. C.}",

note = "The authors thank the Deutsche Forschungsgemeinschaft (DFG) for funding this research (grant no. KU 2738/3-2). KMJS is supported by the German Federal Ministry of Education and Research (IMMUNOMOD research group, 01KI2014). M.C.G. acknowledges financial support from the European Research Council under the European Union{\textquoteright}s Horizon 2020 Framework Programme (ERC StG ABLASE, 640012) and from the Alexander von Humboldt Stiftung (Humboldt-Professorship).",

year = "2020",

month = dec,

day = "9",

doi = "10.1002/adom.202001553",

language = "English",

volume = "Early View",

journal = "Advanced Optical Materials",

issn = "2195-1071",

publisher = "John Wiley & Sons, Ltd",

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TY - JOUR

T1 - Narrow stimulated resonance Raman scattering and WGM lasing in small conjugated polymer particles for live cell tagging and tracking

AU - Haehnle, Bastian

AU - Lamla,, Markus

AU - Sparrer,, Konstantin M. J

AU - Gather, Malte C.

AU - Kuehne, Alexander J. C.

N1 - The authors thank the Deutsche Forschungsgemeinschaft (DFG) for funding this research (grant no. KU 2738/3-2). KMJS is supported by the German Federal Ministry of Education and Research (IMMUNOMOD research group, 01KI2014). M.C.G. acknowledges financial support from the European Research Council under the European Union’s Horizon 2020 Framework Programme (ERC StG ABLASE, 640012) and from the Alexander von Humboldt Stiftung (Humboldt-Professorship).

PY - 2020/12/9

Y1 - 2020/12/9

N2 - Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here we present, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles as a new method for generating narrow emission as an alternative to WGM-based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. We discuss the synthesis of monodisperse micron-sized poly(fluorene-co-divinylbenzene) particles andcharacterize their WGM and SRRS emission. Finally, we showcase how these particles and their emission can be employed in live cell imaging and tagging.

AB - Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here we present, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles as a new method for generating narrow emission as an alternative to WGM-based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. We discuss the synthesis of monodisperse micron-sized poly(fluorene-co-divinylbenzene) particles andcharacterize their WGM and SRRS emission. Finally, we showcase how these particles and their emission can be employed in live cell imaging and tagging.

KW - live cell imaging

KW - Conjugated polymer particles

KW - Laser emission

KW - Whispering gallery mode

U2 - 10.1002/adom.202001553

DO - 10.1002/adom.202001553

M3 - Article

SN - 2195-1071

VL - Early View

JO - Advanced Optical Materials

JF - Advanced Optical Materials

M1 - 2001553

ER -

Narrow stimulated resonance Raman scattering and WGM lasing in small conjugated polymer particles for live cell tagging and tracking

Abstract

Keywords

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Projects

H2020 ERC Starting Grant ABLASE: H2020 ERC Starting Grant ABLASE (Grant Agreement No. 640012)

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