Myogenin in model pufferfish species: Comparative genomic analysis and thermal plasticity of expression during early development

J M O Fernandes, M G MacKenzie, P A Wright, S L Steele, Y Suzuki, J R Kinghorn, Ian Alistair Johnston

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Myogenin (Myog) is a muscle-specific basic helix-loop-helix transcription factor that plays an essential role in the specification and differentiation of myoblasts. The myogenin genes from the tiger pufferfish, Takifugu rubripes, and green-spotted pufferfish, Tetraodon nigroviridis, were cloned and a comparative genomic analysis performed. The gene encoding myogenin is composed of three exons and has a relatively similar genomic structure in T rubripes, T nigroviridis and human. Introns 1 and 2 were approximately 2-fold and 8-fold longer respectively in human than pufferfish. Myogenin is located in a 100 kb region of conserved synteny between these organisms, corresponding to chromosome 1 in human, chromosome 11 in T nigroviridis and scaffold 208 in T rubripes. Pufferfish myogenin contained a serine-rich region at the carboxyl terminus that is highly conserved amongst teleosts. During embryonic development of T rubripes, myogenin was expressed in a rostral-caudal gradient in the developing somites and subsequently during the pharyngula period in the pectoral fin bud primordia, jaw muscles and extraocular muscle precursors. In T rubripes, the time required to form a somite pair during the linear phase of somitogenesis ( somite-interval) was 122 min, 97 min and 50 min in embryos incubated at 15, 18 and 21 degrees C, respectively. Myogenin mRNA transcripts were quantified using qPCR and normalised to the highest level of expression. Peak myogenin expression occurred later with respect to developmental stage (standardised using somite-intervals) and was over 2-fold higher at 21 degrees C than at either 18 or 15 degrees C. Changes in the relative timing and intensity of myogenin expression are a potential mechanism for explaining thermal plasticity of muscle phenotype in larvae via effects on the differentiation programme. (c) 2005 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)35-45
Number of pages11
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume1
Issue number1
DOIs
Publication statusPublished - Mar 2006

Keywords

  • Takifugu rubripes
  • Tetraodon nigroviridis
  • myogenin
  • myogenic factor 4
  • development
  • myogenesis
  • thermal plasticity
  • temperature
  • MUSCLE-SPECIFIC EXPRESSION
  • HERRING CLUPEA-HARENGUS
  • TROUT ONCORHYNCHUS-MYKISS
  • ATLANTIC SALMON
  • SKELETAL-MUSCLE
  • RAINBOW-TROUT
  • DIFFERENTIAL EXPRESSION
  • EMBRYONIC TEMPERATURE
  • TRANSCRIPTION FACTORS
  • MYOTOMAL MUSCULATURE

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