Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

Janine T Bossé; Yanwen Li; Oystein Angen; Lucy A Weinert; Roy R Chaudhuri; Matt T Holden; Susanna M Williamson; Duncan J Maskell; Alexander W Tucker; Brendan W Wren; Andrew N Rycroft; Paul R Langford; BRaDP1T Consortium; Matthew Holden

doi:10.1128/JCM.00685-14

Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

Janine T Bossé, Yanwen Li, Oystein Angen, Lucy A Weinert, Roy R Chaudhuri, Matt T Holden, Susanna M Williamson, Duncan J Maskell, Alexander W Tucker, Brendan W Wren, Andrew N Rycroft, Paul R Langford, BRaDP1T Consortium, Matthew Holden

Research output: Contribution to journal › Article › peer-review

Abstract

An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

Original language	English
Pages (from-to)	2380-2385
Number of pages	6
Journal	Journal of Clinical Microbiology
Early online date	23 Apr 2014
DOIs	https://doi.org/10.1128/JCM.00685-14
Publication status	Published - Jul 2014

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10.1128/JCM.00685-14

Holden_2014_JCM_Multiplex
Copyright © 2014 Bossé et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license. https://creativecommons.org/licenses/by/3.0/
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Bossé, J. T., Li, Y., Angen, O., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., Williamson, S. M., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R., BRaDP1T Consortium, & Holden, M. (2014). Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12. Journal of Clinical Microbiology, 2380-2385. https://doi.org/10.1128/JCM.00685-14

@article{aa9f986379c44589985447de330cd3c4,

title = "Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12",

abstract = "An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.",

author = "Boss{\'e}, {Janine T} and Yanwen Li and Oystein Angen and Weinert, {Lucy A} and Chaudhuri, {Roy R} and Holden, {Matt T} and Williamson, {Susanna M} and Maskell, {Duncan J} and Tucker, {Alexander W} and Wren, {Brendan W} and Rycroft, {Andrew N} and Langford, {Paul R} and {BRaDP1T Consortium} and Matthew Holden",

note = "This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.",

year = "2014",

month = jul,

doi = "10.1128/JCM.00685-14",

language = "English",

pages = "2380--2385",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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Bossé, JT, Li, Y, Angen, O, Weinert, LA, Chaudhuri, RR, Holden, MT, Williamson, SM, Maskell, DJ, Tucker, AW, Wren, BW, Rycroft, AN, Langford, PR, BRaDP1T Consortium & Holden, M 2014, 'Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12', Journal of Clinical Microbiology, pp. 2380-2385. https://doi.org/10.1128/JCM.00685-14

TY - JOUR

T1 - Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

AU - Bossé, Janine T

AU - Li, Yanwen

AU - Angen, Oystein

AU - Weinert, Lucy A

AU - Chaudhuri, Roy R

AU - Holden, Matt T

AU - Williamson, Susanna M

AU - Maskell, Duncan J

AU - Tucker, Alexander W

AU - Wren, Brendan W

AU - Rycroft, Andrew N

AU - Langford, Paul R

AU - BRaDP1T Consortium

AU - Holden, Matthew

N1 - This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food and Rural Af-fairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.

PY - 2014/7

Y1 - 2014/7

N2 - An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

AB - An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovar 3, 6 and 8 Actinobacillus pleuropneumoniae isolates is described. The new primers eliminate an aberrant serovar 3 indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for detection of serovars 1-3, 5-8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

U2 - 10.1128/JCM.00685-14

DO - 10.1128/JCM.00685-14

M3 - Article

C2 - 24759717

SN - 0095-1137

SP - 2380

EP - 2385

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

ER -

Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12

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