Monoclonal antibodies to the C4 region of human immunodeficiency virus type 1 gp120: use in topological analysis of a CD4 binding site

J A McKeating, J P Moore, M Ferguson, H S Marsden, S Graham, J W Almond, D J Evans, R A Weiss

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28 Citations (Scopus)

Abstract

We have raised antisera and monoclonal antibodies (MAbs) to the C4 region of HIV-1 gp120, using an antigen chimaera of poliovirus as immunogen. These MAbs and sera, together with MAbs to the same region raised by other methods, fall into three groups defined by their abilities to bind to recombinant gp120 and/or the immunogenic peptide. In some cases, the amino acids recognized by the MAbs have been identified by pep-scan and by solution phase peptide inhibition of binding to recombinant gp120. Our results indicate that the amino acids WQEVGKAMYA are exposed on the surface of recombinant gp120. Antibodies to these amino acids on recombinant gp120 compete for soluble CD4 binding in vitro, but only weakly neutralize HIV.
Original languageEnglish
Pages (from-to)451-9
Number of pages9
JournalAIDS Research and Human Retroviruses
Volume8
Issue number4
Publication statusPublished - Apr 1992

Keywords

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, CD4
  • Antigens, Viral
  • Base Sequence
  • Binding Sites
  • Binding, Competitive
  • DNA, Viral
  • Epitopes
  • HIV Envelope Protein gp120
  • HIV-1
  • Molecular Sequence Data
  • Neutralization Tests
  • Poliovirus

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