Projects per year
Abstract
Biomolecular complexes are often multimers fueling the demand for methods that allow unraveling their composition and geometric arrangement. Pulse electron paramagnetic resonance (EPR) spectroscopy is increasingly applied for retrieving geometric information on the nanometer scale. The emerging RIDME (relaxation-induced dipolar modulation enhancement) technique offers improved sensitivity in distance experiments involving metal centers (e.g. on metalloproteins or proteins labelled with metal ions). Here, a mixture of a spin labelled ligand with increasing amounts of paramagnetic CuII ions allowed accurate quantification of ligand-metal binding in the model complex formed. The distance measurement was highly accurate and critical aspects for identifying multimerization could be identified. The potential to quantify binding in addition to the high-precision distance measurement will further increase the scope of EPR applications.
Original language | English |
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Pages (from-to) | 2318-2321 |
Number of pages | 5 |
Journal | ChemPhysChem |
Volume | 18 |
Issue number | 17 |
Early online date | 1 Aug 2017 |
DOIs | |
Publication status | Published - 6 Sept 2017 |
Keywords
- Complexes
- Distance measurements
- EPR spectroscopy
- Metalloproteins
- Multimers
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Dive into the research topics of 'Monitoring complex formation by relaxation-induced pulse electron paramagnetic resonance distance measurements'. Together they form a unique fingerprint.Projects
- 3 Finished
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State of the art pulse EPR instrumentati: State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules
Smith, G. M. (PI), Bode, B. E. (CoI), Naismith, J. (CoI), Schiemann, O. (CoI) & White, M. (CoI)
1/09/12 → 31/08/17
Project: Standard
Datasets
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Data Underpinning: Monitoring Complex Formation by Relaxation-Induced Pulse Electron Paramagnetic Resonance Distance Measurements
Giannoulis, A. (Creator), Oranges, M. (Creator) & Bode, B. E. (Creator), University of St Andrews, 30 Aug 2017
DOI: 10.17630/8a0dc118-48b0-46a0-bc9f-a6bbc3f970fb
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