Molecular evidence for three genetic species of Dipteryx in the Peruvian Amazon

Carmen Garcia-Davila*, David Aldana Gomero, Jean François Renno, Rossana Diaz Soria, Gabriel Hidalgo Pizango, Gerardo Flores Llampazo, Diana Castro-Ruiz, Eduardo Mejia de Loayza, Carlos Angulo Chavez, Malte Mader, Niklas Tysklind, Kathelyn Paredes-Villanueva, Dennis del Castillo Torres, Bernd Degen, Eurídice N. Honorio Coronado

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

There is a high international demand for timber from the genus Dipteryx, or “shihuahuaco” as it is known in Peru. Developing tools that allow the identification and discrimination of Dipteryx species is therefore important for supporting management of natural populations and to underpin legal trade of its timber. The objective of this study was the molecular characterization of Dipteryx species in the Peruvian Amazonia. Two plastid regions (cpDNA: trnH–psbA and matK) were sequenced and 11 microsatellite markers (nDNA) were genotyped for 32 individuals identified as Dipteryx charapilla, D. micrantha morphotype 1 and D. micrantha morphotype 2. Using the concatenated sequences of the plastid genes, we identified ten haplotypes that were not shared between the species or between the D. micrantha morphotypes. Haplotypic diversity was greater in D. micrantha morphotype 2 and D. charapilla than in D. micrantha morphotype 1, which presented only one haplotype with a wide distribution in Peru. The microsatellites allowed the discrimination of the same three clades and identified diagnostic alleles for each clade. These results allowed us to demonstrate that the two morphotypes of D. micrantha are different at both the plastid and nuclear markers, which supports the existence of three genetically distinct species in Peru. This study provides information for the genetic discrimination of Dipteryx species and emphasises the importance of conserving the genetic variability of this genus in the Peruvian Amazonia.

Original languageEnglish
Number of pages11
JournalGenetica
Volume148
Early online date19 Dec 2019
DOIs
Publication statusPublished - 1 Feb 2020

Keywords

  • D. micrantha
  • Dipteryx charapilla
  • Genetic diversity
  • Microsatellites
  • Sequencing
  • Shihuahuaco

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