Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a

Jose Luis Ruiz-Barba*, Belen Floriano, Antonio Maldonado-Barragan, Rufino Jimenez-Diaz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The complete 21,344-bp DNA sequence of the bacteriocin-encoding plasmid pEF1 from Enterococcusfaecium 6TI a was determined. Thirty-four putative open reading frames which could code for proteins longer than 42 amino acids were found. Those included the structural genes encoding for the previously described bacteriocins enterocin I and J (also named as enterocins L50A and L50B). After comparison to sequences in public databases, analysis of the gene organization of pEFI suggests a modular structure with three different functional domains: the replication region, the bacteriocin region and the mobilization plus UV-resistance region. This genetic mosaic structure most probably evolved through recombination events promoted by transposable elements. The hypothesis that the bacteriocin cluster on pEF1 could act as a functional plasmid stabilization module in E. faecium 6T1a is discussed. (c) 2006 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)175-181
Number of pages7
JournalPlasmid
Volume57
Issue number2
DOIs
Publication statusPublished - Mar 2007

Keywords

  • bacteriocinogenic plasmid
  • plasmid stability
  • Enterococcus faecium
  • nucleotide sequence
  • annotation
  • LACTIC-ACID BACTERIA
  • CONJUGATIVE PLASMID
  • PHEROMONE RESPONSE
  • GENETIC-ANALYSIS
  • ENTEROCINS L50A
  • FAECALIS
  • SEQUENCE
  • DETERMINANT
  • IMMUNITY
  • AS-48

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