Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a

Jose Luis Ruiz-Barba; Belen Floriano; Antonio Maldonado-Barragan; Rufino Jimenez-Diaz

doi:10.1016/j.plasmid.2006.06.003

Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a

Jose Luis Ruiz-Barba^*, Belen Floriano, Antonio Maldonado-Barragan, Rufino Jimenez-Diaz

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

The complete 21,344-bp DNA sequence of the bacteriocin-encoding plasmid pEF1 from Enterococcusfaecium 6TI a was determined. Thirty-four putative open reading frames which could code for proteins longer than 42 amino acids were found. Those included the structural genes encoding for the previously described bacteriocins enterocin I and J (also named as enterocins L50A and L50B). After comparison to sequences in public databases, analysis of the gene organization of pEFI suggests a modular structure with three different functional domains: the replication region, the bacteriocin region and the mobilization plus UV-resistance region. This genetic mosaic structure most probably evolved through recombination events promoted by transposable elements. The hypothesis that the bacteriocin cluster on pEF1 could act as a functional plasmid stabilization module in E. faecium 6T1a is discussed. (c) 2006 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	175-181
Number of pages	7
Journal	Plasmid
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/j.plasmid.2006.06.003
Publication status	Published - Mar 2007

Keywords

bacteriocinogenic plasmid
plasmid stability
Enterococcus faecium
nucleotide sequence
annotation
LACTIC-ACID BACTERIA
CONJUGATIVE PLASMID
PHEROMONE RESPONSE
GENETIC-ANALYSIS
ENTEROCINS L50A
FAECALIS
SEQUENCE
DETERMINANT
IMMUNITY
AS-48

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@article{30f91724aecd431cbae6b47ab0ad27b8,

title = "Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a",

abstract = "The complete 21,344-bp DNA sequence of the bacteriocin-encoding plasmid pEF1 from Enterococcusfaecium 6TI a was determined. Thirty-four putative open reading frames which could code for proteins longer than 42 amino acids were found. Those included the structural genes encoding for the previously described bacteriocins enterocin I and J (also named as enterocins L50A and L50B). After comparison to sequences in public databases, analysis of the gene organization of pEFI suggests a modular structure with three different functional domains: the replication region, the bacteriocin region and the mobilization plus UV-resistance region. This genetic mosaic structure most probably evolved through recombination events promoted by transposable elements. The hypothesis that the bacteriocin cluster on pEF1 could act as a functional plasmid stabilization module in E. faecium 6T1a is discussed. (c) 2006 Elsevier Inc. All rights reserved.",

keywords = "bacteriocinogenic plasmid, plasmid stability, Enterococcus faecium, nucleotide sequence, annotation, LACTIC-ACID BACTERIA, CONJUGATIVE PLASMID, PHEROMONE RESPONSE, GENETIC-ANALYSIS, ENTEROCINS L50A, FAECALIS, SEQUENCE, DETERMINANT, IMMUNITY, AS-48",

author = "Ruiz-Barba, {Jose Luis} and Belen Floriano and Antonio Maldonado-Barragan and Rufino Jimenez-Diaz",

year = "2007",

month = mar,

doi = "10.1016/j.plasmid.2006.06.003",

language = "English",

volume = "57",

pages = "175--181",

journal = "Plasmid",

issn = "0147-619X",

publisher = "Academic Press/Elsevier ",

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TY - JOUR

T1 - Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a

AU - Ruiz-Barba, Jose Luis

AU - Floriano, Belen

AU - Maldonado-Barragan, Antonio

AU - Jimenez-Diaz, Rufino

PY - 2007/3

Y1 - 2007/3

N2 - The complete 21,344-bp DNA sequence of the bacteriocin-encoding plasmid pEF1 from Enterococcusfaecium 6TI a was determined. Thirty-four putative open reading frames which could code for proteins longer than 42 amino acids were found. Those included the structural genes encoding for the previously described bacteriocins enterocin I and J (also named as enterocins L50A and L50B). After comparison to sequences in public databases, analysis of the gene organization of pEFI suggests a modular structure with three different functional domains: the replication region, the bacteriocin region and the mobilization plus UV-resistance region. This genetic mosaic structure most probably evolved through recombination events promoted by transposable elements. The hypothesis that the bacteriocin cluster on pEF1 could act as a functional plasmid stabilization module in E. faecium 6T1a is discussed. (c) 2006 Elsevier Inc. All rights reserved.

AB - The complete 21,344-bp DNA sequence of the bacteriocin-encoding plasmid pEF1 from Enterococcusfaecium 6TI a was determined. Thirty-four putative open reading frames which could code for proteins longer than 42 amino acids were found. Those included the structural genes encoding for the previously described bacteriocins enterocin I and J (also named as enterocins L50A and L50B). After comparison to sequences in public databases, analysis of the gene organization of pEFI suggests a modular structure with three different functional domains: the replication region, the bacteriocin region and the mobilization plus UV-resistance region. This genetic mosaic structure most probably evolved through recombination events promoted by transposable elements. The hypothesis that the bacteriocin cluster on pEF1 could act as a functional plasmid stabilization module in E. faecium 6T1a is discussed. (c) 2006 Elsevier Inc. All rights reserved.

KW - bacteriocinogenic plasmid

KW - plasmid stability

KW - Enterococcus faecium

KW - nucleotide sequence

KW - annotation

KW - LACTIC-ACID BACTERIA

KW - CONJUGATIVE PLASMID

KW - PHEROMONE RESPONSE

KW - GENETIC-ANALYSIS

KW - ENTEROCINS L50A

KW - FAECALIS

KW - SEQUENCE

KW - DETERMINANT

KW - IMMUNITY

KW - AS-48

U2 - 10.1016/j.plasmid.2006.06.003

DO - 10.1016/j.plasmid.2006.06.003

M3 - Article

SN - 0147-619X

VL - 57

SP - 175

EP - 181

JO - Plasmid

JF - Plasmid

IS - 2

ER -

Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a

Abstract

Keywords

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