Molecular analysis of intraspecific variation between building and 'wild' isolates of Serpula lacrymans and their relatedness to S. himantioides

NA White; PK Dehal; JM Duncan; NA Williams; JS Gartland; JW Palfreyman; DEL Cooke

doi:10.1017/s0953756201003781

Molecular analysis of intraspecific variation between building and 'wild' isolates of Serpula lacrymans and their relatedness to S. himantioides

NA White, PK Dehal, JM Duncan, NA Williams, JS Gartland, JW Palfreyman, DEL Cooke

School of Chemistry

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Relationships between Serpula lacrymans and S. himantioides isolates of ‘wild’ Himalayan woodland and building (European and Australian) origin were examined using RAPD–PCR and ITS rDNA sequence comparison. GelCompar analysis of the genetic fingerprints of eighteen isolates using four RAPD-primer combinations clearly distinguished S. lacrymans and S. himantioides. Little intraspecific variation was observed in S. lacrymans (including isolates of Himalayan woodland origin), relative to S. himantioides. Despite previously reported differences in growth rates and decay capabilities of ‘wild’ Himalayan woodland S. lacrymans isolates and those from buildings, RAPD fingerprints and ITS sequences indicated they were genetically similar. Interspecific ITS sequence polymorphisms were consistent with other studies and clearly demonstrated S. himantioides and S. lacrymans were distinct taxa. Evolutionary questions regarding the origins of S. lacrymans are discussed.

Original language	Undefined/Unknown
Pages (from-to)	447—452
Journal	Mycological Research
Volume	105
Issue number	4
DOIs	https://doi.org/10.1017/s0953756201003781
Publication status	Published - 1 Apr 2001

Access to Document

10.1017/s0953756201003781

https://doi.org/10.1017/S0953756201003781

Cite this

@article{d391e7e63f9342e5b2a5b7afc19c34bf,

title = "Molecular analysis of intraspecific variation between building and 'wild' isolates of Serpula lacrymans and their relatedness to S. himantioides",

abstract = "Relationships between Serpula lacrymans and S. himantioides isolates of {\textquoteleft}wild{\textquoteright} Himalayan woodland and building (European and Australian) origin were examined using RAPD–PCR and ITS rDNA sequence comparison. GelCompar analysis of the genetic fingerprints of eighteen isolates using four RAPD-primer combinations clearly distinguished S. lacrymans and S. himantioides. Little intraspecific variation was observed in S. lacrymans (including isolates of Himalayan woodland origin), relative to S. himantioides. Despite previously reported differences in growth rates and decay capabilities of {\textquoteleft}wild{\textquoteright} Himalayan woodland S. lacrymans isolates and those from buildings, RAPD fingerprints and ITS sequences indicated they were genetically similar. Interspecific ITS sequence polymorphisms were consistent with other studies and clearly demonstrated S. himantioides and S. lacrymans were distinct taxa. Evolutionary questions regarding the origins of S. lacrymans are discussed.",

author = "NA White and PK Dehal and JM Duncan and NA Williams and JS Gartland and JW Palfreyman and DEL Cooke",

year = "2001",

month = apr,

day = "1",

doi = "10.1017/s0953756201003781",

language = "Undefined/Unknown",

volume = "105",

pages = "447—452",

journal = "Mycological Research",

issn = "0953-7562",

publisher = "Cambridge University Press",

number = "4",

}

TY - JOUR

T1 - Molecular analysis of intraspecific variation between building and 'wild' isolates of Serpula lacrymans and their relatedness to S. himantioides

AU - White, NA

AU - Dehal, PK

AU - Duncan, JM

AU - Williams, NA

AU - Gartland, JS

AU - Palfreyman, JW

AU - Cooke, DEL

PY - 2001/4/1

Y1 - 2001/4/1

N2 - Relationships between Serpula lacrymans and S. himantioides isolates of ‘wild’ Himalayan woodland and building (European and Australian) origin were examined using RAPD–PCR and ITS rDNA sequence comparison. GelCompar analysis of the genetic fingerprints of eighteen isolates using four RAPD-primer combinations clearly distinguished S. lacrymans and S. himantioides. Little intraspecific variation was observed in S. lacrymans (including isolates of Himalayan woodland origin), relative to S. himantioides. Despite previously reported differences in growth rates and decay capabilities of ‘wild’ Himalayan woodland S. lacrymans isolates and those from buildings, RAPD fingerprints and ITS sequences indicated they were genetically similar. Interspecific ITS sequence polymorphisms were consistent with other studies and clearly demonstrated S. himantioides and S. lacrymans were distinct taxa. Evolutionary questions regarding the origins of S. lacrymans are discussed.

AB - Relationships between Serpula lacrymans and S. himantioides isolates of ‘wild’ Himalayan woodland and building (European and Australian) origin were examined using RAPD–PCR and ITS rDNA sequence comparison. GelCompar analysis of the genetic fingerprints of eighteen isolates using four RAPD-primer combinations clearly distinguished S. lacrymans and S. himantioides. Little intraspecific variation was observed in S. lacrymans (including isolates of Himalayan woodland origin), relative to S. himantioides. Despite previously reported differences in growth rates and decay capabilities of ‘wild’ Himalayan woodland S. lacrymans isolates and those from buildings, RAPD fingerprints and ITS sequences indicated they were genetically similar. Interspecific ITS sequence polymorphisms were consistent with other studies and clearly demonstrated S. himantioides and S. lacrymans were distinct taxa. Evolutionary questions regarding the origins of S. lacrymans are discussed.

U2 - 10.1017/s0953756201003781

DO - 10.1017/s0953756201003781

M3 - Article

SN - 0953-7562

VL - 105

SP - 447—452

JO - Mycological Research

JF - Mycological Research

IS - 4

ER -