Projects per year
Abstract
Standard Raman spectroscopy (SRS) is a noninvasive technique that is used in the biomedical field to discriminate between normal and cancer cells. However, the presence of a strong fluorescence background detracts from the use of SRS in real-time clinical applications. Recently, we have reported a novel modulated Raman spectroscopy (MRS) technique to extract the Raman spectra from the background. In this paper, we present the first application of MRS to the identification of human urothelial cells (SV-HUC-1) and bladder cancer cells (MGH) in urine samples. These results are compared to those obtained by SRS. Classification using the principal component analysis clearly shows thatMRS allows discrimination between Raman spectra of SV-HUC-1 andMGH cells with high sensitivity (98%) and specificity (95%). MRS is also used to distinguish between SV-HUC-1 and MGH cells after exposure to urine for up to 6 h.We observe a marked change in the MRS of SV-HUC-1 and MGH cells with time in urine, indicating that the conditions of sample collection will be important for the application of this methodology to clinical urine samples.
Original language | English |
---|---|
Article number | 037002 |
Number of pages | 7 |
Journal | Journal of Biomedical Optics |
Volume | 16 |
Issue number | 3 |
DOIs | |
Publication status | Published - Mar 2011 |
Keywords
- Raman spectroscopy
- Modulation spectroscopy
- Bladder cancer
Fingerprint
Dive into the research topics of 'Modulated Raman spectroscopy for enhanced identification of bladder tumor cells in urine samples'. Together they form a unique fingerprint.Projects
- 1 Finished
-
EPSRC Postdoctoral Research Fellowship: EPSRC Postdoctoral Fellowship
De Luca, A. C. (PI)
1/10/10 → 29/02/12
Project: Fellowship