Micro-evolution of Burkholderia pseudomallei during an acute infection

Direk Limmathurotsakul; Matthew T G Holden; Paul Coupland; Erin P Price; Narisara Chantratita; Vanaporn Wuthiekanun; Premjit Amornchai; Julian Parkhill; Sharon J Peacock

doi:10.1128/JCM.01219-14

Micro-evolution of Burkholderia pseudomallei during an acute infection

Direk Limmathurotsakul, Matthew T G Holden, Paul Coupland, Erin P Price, Narisara Chantratita, Vanaporn Wuthiekanun, Premjit Amornchai, Julian Parkhill, Sharon J Peacock

Research output: Contribution to journal › Article › peer-review

Abstract

We used whole-genome sequencing to evaluate sixty-nine independent colonies of Burkholderia pseudomallei isolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for rapid in vivo diversification of B. pseudomallei after inoculation, and systemic spread of a heterogeneous population.

Original language	English
Journal	Journal of Clinical Microbiology
Early online date	25 Jun 2014
DOIs	https://doi.org/10.1128/JCM.01219-14
Publication status	Published - 2014

Keywords

Melioidosis
Burkholderia pseudomallei
Whole genome sequencing
Within-host evolution

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10.1128/JCM.01219-14

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title = "Micro-evolution of Burkholderia pseudomallei during an acute infection",

abstract = "We used whole-genome sequencing to evaluate sixty-nine independent colonies of Burkholderia pseudomallei isolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for rapid in vivo diversification of B. pseudomallei after inoculation, and systemic spread of a heterogeneous population.",

keywords = "Melioidosis, Burkholderia pseudomallei, Whole genome sequencing, Within-host evolution",

author = "Direk Limmathurotsakul and Holden, {Matthew T G} and Paul Coupland and Price, {Erin P} and Narisara Chantratita and Vanaporn Wuthiekanun and Premjit Amornchai and Julian Parkhill and Peacock, {Sharon J}",

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journal = "Journal of Clinical Microbiology",

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AU - Limmathurotsakul, Direk

AU - Holden, Matthew T G

AU - Coupland, Paul

AU - Price, Erin P

AU - Chantratita, Narisara

AU - Wuthiekanun, Vanaporn

AU - Amornchai, Premjit

AU - Parkhill, Julian

AU - Peacock, Sharon J

PY - 2014

Y1 - 2014

N2 - We used whole-genome sequencing to evaluate sixty-nine independent colonies of Burkholderia pseudomallei isolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for rapid in vivo diversification of B. pseudomallei after inoculation, and systemic spread of a heterogeneous population.

AB - We used whole-genome sequencing to evaluate sixty-nine independent colonies of Burkholderia pseudomallei isolated from seven body sites of a patient with acute disseminated melioidosis. Fourteen closely related genotypes were found, providing evidence for rapid in vivo diversification of B. pseudomallei after inoculation, and systemic spread of a heterogeneous population.

KW - Melioidosis

KW - Burkholderia pseudomallei

KW - Whole genome sequencing

KW - Within-host evolution

U2 - 10.1128/JCM.01219-14

DO - 10.1128/JCM.01219-14

M3 - Article

C2 - 24966357

SN - 0095-1137

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

ER -

Micro-evolution of Burkholderia pseudomallei during an acute infection

Abstract

Keywords

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